MOLECULAR DETECTION OF RIFAMPICIN AND ISONIAZID RESISTANCE IN CULTURE ISOLATES OF NEWLY DIAGNOSED TB PATIENTS
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2014, Vol 3, Issue 2
Abstract
Introduction: Multidrug-resistant tuberculosis (MDR-TB) is an emerging public health problem in many regions of the world, particularly in developing nations. Accurate and rapid diagnosis is essential in the management of MDR-TB, not only to optimize treatment but also to prevent transmission. Aims: To evaluate drug resistance in culture isolates by conventional and molecular methods and detect drug resistance gene in MDR-TB patients. Material and Method: 100 newly diagnosed pulmonary tuberculosis (TB) diagnosed patients attending TB Clinic, Gandhi Hospital, Secunderabad were included in the study. Two sputum samples collected from the patients were subjected to sputum microscopy, culture, Drug Susceptibility Testing (DST). Geno Type Mycobacterium Tuberculosis Drug Resistance (MTBDR) plus assay was done on the culture isolates to detect Rifampicin and Isoniazid (INH) resistance. Results: Out of 100 samples, 48 % smear positivity by Ziehl Neelsen (ZN) method, 51 % culture positivity on LJ medium,11.7% multi drug resistance for Rifampicin and Isoniazid with conventional drug susceptibility – Proportion method,17.6 % drug resistance by molecular method – Geno Type MTBDR plus was observed. Among the 4 Rifampicin (Rif) resistant isolates 2isolates showed mutation (mut) at D516V and in other 2 isolates only wild type (WT) was missing but no mut was seen . In the 1 Isoniazid (INH) resistant isolate WT was missing, but no mutation was seen. Among the 4 Rif +INH resistance all showed mut at S531L for RIF and at S315T1. Conclusion: The Genotype MTBDR assay is a rapid and reliable tool for the routine direct detection of MTB strains and of strains resistant to INH and RIF in smear positive, highly infectious patients. The rapid turn around time of the test enables the optimization of the therapy of these patients before confirmatory culture results are available. The test does not require viable organisms and thus reduces the biohazard risk in the laboratory.
Authors and Affiliations
Vanisree R| Dept. of Microbiology, Osmania medical college, Hyderabad, Andhra Pradesh, India, Kavitha Latha M| Dept. of Microbiology, Gandhi medical college, Secunderabad, Andhra Pradesh, India, Neelima A| Dept of Microbiology, Mediciti Institute of Medical Sciences, Andhra Pradesh, India, Corresponding author email: neelimasudharshan@gmail.com, Prasanti| Dept. of Microbiology, Gandhi medical college, Secunderabad, Andhra Pradesh, India
Keywords
Related Articles
- EP ID EP11177
- DOI 10.5958/j.2319-5886.3.2.078
- Views 311
- Downloads 16
Association between sodium bicarbonate consumption and human health: A systematic review
Sodium bicarbonate or baking soda is a chemical compound dissolved in water which is widely used as an additive in foods and mineral water and as a medicine. In Iran, due to the introduction of harmful effects of this c...
ISOLATED MAJOR AORTOPULMONARY COLLATERAL ARTERY CAUSING CCF IN A NEWBORN: A CASE REPORT
Major Aortopulmonary collateral artery (MAPCA) is an anamolous vascular connection in between aorta or one of its main branches and pulmonary artery. It is single or multiple in which case it’s called multiple anamol...
Pseudoepitheliomatous hyperplasia: A review of oral lesions
Pseudoepitheliomatous hyperplasia is a histological reactive pattern of surface epithelium in response to various stimuli from the underlying connective tissue which frequently mimics squamous cell carcinoma. So, differ...
Determination of the Relationship between the Quality of Working Life and Tendency for Continuing Education in the Nurses working in Selected Hospitals of Isfahan Medical University
In the past, the main role of nurses was providing care and comfort during nursing duties. However, today due to the changes in the health-related systems, more emphasis is put on the health promotion, disease preventio...
A STUDY ON SERUM ENZYME LEVELS IN VARIOUS LIVER DISEASES
Patients with chronic liver diseases are asymptomatic or have only vague non-specific symptoms. Effective medical treatments for chronic liver disease (before cirrhosis is established) are becoming increasingly availab...