Multi-Epitope Cluster Ep85B within the Mycobacterial Protein Ag85B Elicits Cell-Mediated and Humoral Responses in Mice
Journal Title: Turkish Journal of Immunology - Year 2018, Vol 6, Issue 3
Abstract
Introduction: Epitope-based vaccines present a rational alternative to conventional concepts of vaccine design, particularly for combating complex infectious agents such as tuberculosis (TB). We have previously identified the multi-epitope cluster Ep85B, a linear 28 amino acid peptide within the mycobacterial Ag85B protein, which is capable of eliciting CD4+ and memory CD4+CD45RO+ T-cell populations in vitro in human whole blood cells. In this report, we investigated the suitability of Ep85B for animal immunizations, and endeavored to demonstrate whether this epitope harbors the potential to induce both cell-mediated and humoral immune responses in vivo. Materials and Methods: For immunization, BALB/c mice were injected subcutaneously with Ep85B. To test cellular immunity, splenocytes from the immunized mice were cultured in the presence of Ep85B and Th1/Th2 specific cytokine responses were analyzed by cytokine bead array. To test humoral immunity, Ep85B-specific IgG, IgM and IgA responses in serum collected from the immunized mice were determined by ELISA. Results: Cytokine bead array displayed a significant increase of IFN-γ, IL-2 and TNF-α in the culture supernatant of splenocytes from the mice immunized with Ep85B compared to the naïve mice indicating the activation of a mixed Th1/Th2 cells. There was an increase of IgG and IgM antibody levels in the serum of mice immunized with Ep85B confirming the ability of the epitope to elicit humoral responses. Conclusion: In silico predicted Ep85B epitope of mycobacterial Ag85B protein can elicit both cellular and humoral responses in mice. Keywords: Immunoinformatics, epitope vaccine, tuberculosis, Ag85B
Authors and Affiliations
S. M. Touhidul ISLAM, Shabnam ZAMAN, Kawsar KHAN, Muhammad Ikhtear UDDIN, Sajib CHAKRABORTY, Naoshin Sharmin NISHAT, Nabilah IBNAT, Mohammad Murshid ALAM, Taufiqur Rahman BHUIYAN, Firdausi QADRI, Zeba I. SERAJ
Keywords
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- EP ID EP450800
- DOI 10.25002/tji.2018.868
- Views 92
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