MULTIVARIATE CALIBRATION TECHNIQUE FOR THE SPECTROPHOTOMETRIC QUANTIFICATION OF IVERMECTIN IN PHARMACEUTICAL FORMULATION
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 2
Abstract
Objective: The present abstract makes the use of multivariate calibration technique for the quantification of ivermectin in pharmaceutical dosage form. Methods: Multivariate calibration technique is based on the use of linear regression equations, by correlating the relation between concentration and absorbance at seven different selected wavelengths. The λmax of ivermectin was found to be 245 nm. The results were treated statistically. This statistical approach gives optimum results by eliminating the fluctuations arising from the instrumental or experimental conditions. Results: The developed method was validated as per the ICH guidelines and was found to be simple, linear, accurate, precise, and reproducible. The method was found to be linear over a concentration range of 5–15 μg/mL with a correlation coefficient (r2) value of about 0.9998. The limit of detection and quantification were found to be 0.029 and 0.087 μg/mL, respectively. The percentage relative standard deviation for intraday and interday precision was found to be in the range of 0.473–1.373 and 0.301–1.617, respectively. The percentage recovery was found within the range of 97.60–101.80% w/w. Conclusion: The results evidence that a simple, linear, precise, accurate, sensitive, and reproducible multivariate calibration technique has been developed and validated for the quantification of ivermectin in bulk and pharmaceutical formulation.
Authors and Affiliations
MADHAN S, KAVITHA J, LAKSHMI KS
Keywords
Related Articles
- EP ID EP608809
- DOI -
- Views 111
- Downloads 0
EFFECTS OF DRIED LEAF POWDER OF OCIMUM SANCTUM IN HEXAMITA-INFECTED FISH ANABAS TESTUDINEUS
Objective: The prophylactic and antioxidative role of the dried powdered of Ocimum sanctum (Tulasi) was measured in Hexamita-infected fish Anabas testudineus. Method: Four groups of infected fish were kept in four separ...
EVALUATION OF ANTIDIABETIC AND ANTIHYPERLIPIDEMIC EFFECT OF VERNONIA DIVERGENS IN STREPTOZOTOCIN-INDUCED DIABETIC RATS
Objective: The current investigation for antidiabetic activity of the plant Vernonia divergens (DC.) Edgew. has not been reported till date. However, to enlighten the folkloric claim of the plant, the study was carried o...
STUDY OF MULTIELEMENTAL CONCENTRATIONS AND NANO-MICRO STRUCTURAL MORPHOLOGY IN MYRTACEAE FAMILY MEDICINAL PLANTS BY FIELD EMISSION SCANNING ELECTRON MICROSCOPE-ENERGY DISPERSIVE X-RAY SPECTROSCOPY METHOD
Objective: The essential multielemental analysis was carried out in nano- and microscale surface morphology of two useful selected species of Myrtaceae family medicinal plants such as Eucalyptus and Guava using “field em...
DESIGN, SYNTHESIS, MOLECULAR DOCKING, AND EVALUATION OF CHROMONE BASED TETRAZOLE DERIVATIVES
Objectives: The objective of this research work was to design, synthesize, study the molecular docking, and evaluate the antimicrobial activity of some novel substituted 2-(Phenylamino)-3-(1H-tetrazol-5-yl)-4H-chromen-4-...
SYNTHESIS OF NEW BORON COMPOUNDS WITH AMOXICILLIN AND SOME OF ITS METAL COMPLEXES WITH USE THEM IN ANTIBACTERIAL, ASSESSMENT OF HEPATOPROTICTIVE AND KIDNEYACTIVITY, ANTICANCER AND ANTIOXIDANT APPLICATIONS
Objective: New ligand ({7-[2-amino-2-(4-hydroxyphenyl)-acetamido-3,3-dimethyl-6-oxo-2-thia-5-aza bicyclo[3,2,0]heptane-4-carboxylic boric anhydride}) with its Co (II), Ni (II) and Cu (II) complexes. And new mixed ligand...