Nanoparticle Evaluation of Synthetic Palmitoyl-CKKHH As Transfection Reagent for Non-Viral Gene Delivery Vehicle
Journal Title: International Journal of engineering Research and Applications - Year 2018, Vol 8, Issue 1
Abstract
Non-viral delivery systems are relatively safe but inefficient in their current form. The main obstacle in using non-viral gene delivery system approach is to transport the gene of interest in cytoplasm and subsequently entering into cell nucleus. In this research, palmityol-CKKHH and its series have been designed and its ability to form nanoparticle of a stable DNA – lipopeptide complex were evaluated to be used as non-viral gene delivery vehicle. The lipopeptide molecules are composed of alkyl chain of palmitoyl (C-16), and amino acid residues of cysteine (C), lysine (K), and histidine (H). The particle size (nm) and zeta potential () of the complexes were determined with a Zetasizer Nano Series. It was revealed that prolonging incubation time of the complex composing of DNA and Pal-CK2H3 (charge ratio of 1.5) more than 2 hours tend to increase the size up to 300 nm. In addition, increasing DNA concentration up to 40 g (~ 120 nmoles) with lipopeptide (charge ratio of 1.5), the complex size was still relatively stable at less than 400 nm. As the number of lysine residue on lipopeptide is increased, the particle size tends to decreased. However, the particle size is increased as the number of histidine residue on lipopeptide is increased. It was also shown that increasing charge ratio of the nanoparticle complex resulted in an increased zeta potential but lowering the particle size. Transfection efficiency of the nanoparticle on COS-7 had shown that the lipopeptide has potency as non-viral gene delivery vehicle. To conclude, the lipopeptide composing of alkyl chain of palmitoyl and amino acid residues form a nanoparticle and having potential characteristics to be further explored as non-viral gene delivery vehicle.
Authors and Affiliations
Tarwadi . , Sabar Pambudi, Heni Rachmawati, Rahmana Emran Kartasasmita, Sukmadjaja Asyarie, Jalal A. Jazayeri, Colin W. Pouton
Keywords
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