AN OVERVIEW ON HPLC, UPLC AND ITS TROUBLESHOOTING
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 4
Abstract
ABSTRACT HPLC and UPLC Systems take advantage of technological strides made in particle chemistry performance, system optimization, detector design, and data processing and control. When taken together, these achievements have created a stepfunction improvement in chromatographic performance.Although HPLC method development has been improved by advances in column technology and instrumentation, problems still arise. This helps in systematic means of isolating, identifying, andcorrecting many typical problems. The important segments of an HPLC and UPLC system are the same, whether you use a modular system or a more sophisticated unit except the column characteristics. Problems affecting overall system performance can arise in each component. Solutions to these problems are presented in easy-to-use tables. Keywords: Monolithic columns, Baseline drift, Shouldering peaks, Fronting peaks, Inline filter, Radio Interference, etc.
Authors and Affiliations
Nidhi Nk*, Neelam I, AjithaA, Uma Maheswar Rao V
Keywords
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