ANALYTICAL PROCESS OF DRUGS BY ULTRAVIOLET (UV) SPECTROSCOPY – A REVIEW
Journal Title: Int J of Preclin & Pharm Res - Year 2012, Vol 2, Issue 2
Abstract
ABSTRACT The pharmaceutical analysis comprises the procedures necessary to determine the “identity, strength, quality and purity” of such compounds. It also includes the analysis of raw material and intermediates during manufacturing process of drugs. Spectrophotometry is generally preferred especially by small-scale industries as the cost of the equipment is less and the maintenance problems are minimal. The method of analysis is based on measuring the absorption of a monochromatic light by colorless compounds in the near ultraviolet path of spectrum (200-380nm). The photometric methods of analysis are based on the Bouger-Lambert-Beer’s law, which establishes that the absorbance of a solution is directly proportional to the concentration of the analyte. The fundamental principle of operation of spectrophotometer covering UV region consists in that light of definite interval of wavelength passes through a cell with solvent and falls on to the photoelectric cell that transforms the radiant energy into electrical energy measured by a galvanometer. Ultraviolet-visible spectroscopy is used to obtain the absorbance spectra of a compound in solution or as a solid. Keywords: Ultraviolet-visible spectroscopy, Bouger-Lambert-Beer’s law, Analytical chemistry.
Authors and Affiliations
R. Gandhimathi*, S. Vijayaraj, M. P. Jyothirmaie
Keywords
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