Antibiogram and Plasmid Profile of Escherichia Coli Isolates in Well Water In Akure, South Western Nigeria
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2013, Vol 3, Issue 7
Abstract
Well water samples sourced from Akure, Ondo State, Nigeria were analyzed using standard and analytical methods to determine the level of divergence of Escherichia coli, in the well water, its antibiogram and the plasmid profile. A total of 400 well water samples were analyzed during the raining and dry season. The mean value of E .coli count obtained ranged from 1.0x10 3 cfu/ml to 3.2x103 cfu/ml. Highest E. coli count was obtained from the sample sourced from Isolo while lowest from FUTA environment. In all, E. coli were isolated from (76) well water sample representing 19% of the 400 sample sourced during the dry season and 108 representing 27% 400 well water sample collected during the raining season. Overall, of 184 E. coli isolates from different well water sourced from different location in Akure were tested against the selected antimicrobial agent. Fifty (50) representing 27.17% of the 184 E. coli isolates showed multiple resistances to (10) antimicrobial agents tested. However, of the ten antimicrobial agents tested, resistance was highest to AM (37) 20.1% follow by SXT (32) 17.4% and CH (31)16.85%. E. coli isolates obtained from Isolo community revealed the highest prevalence of resistance to antimicrobial agent (SXT, CH, S, SP, CPS, AM, AU, and CN). The antimicrobial agents tested were; Sulphamethoxazole (SXT) 30μg, Chloramphenicol (CH) 30μg, Sparfloxacin (SP) 10μg, Ciprofloxacin(CPX)10μg, Amoxcillin (AM)30μg, Augmentin (AU)30μg, Gentamicin (CN)10μg, Pefloxacin(PEF)30μg, Trivid(OFX)10μg, Streptomycin(S)30μg. Plasmid profile analysis of 50 E.coli isolates that showed multiple resistances by agarose gel electrophoresis showed a total of 48 different plasmid bands occurring in various combinations. The microbiological analysis of the well water sample used in this research did not meet the recommended limits and could pose a serious health risk to consumers.
Authors and Affiliations
Bello B. K, Adebolu, T. T
Keywords
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