Determination of kaempferol and quercetin in Xindakang tablet by -cyclodextrin modified micellar electrokinetic capillary chromatography
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 3
Abstract
A method was proposed to determine kaempferol and quercetin in Hippophae rhamnoides L medicinal preparation xindakang tablet by -cyclodextrin modified micellar electrokinetic capillary chromatography. Under the optimized conditions: buffer solution of 20 mmol/L Na2B4O7-KH2PO4 (pH 9.0)-20mmol/L SDS-6mmol/L -CD-5%(v/v) MeCN, applied voltage of 16 kV and injection time of 8s, the two analytes were separated well within 10 minutes. The calibration was linear in the 0.02-0.80 and 0.02-0.70 mg/mL range for kaempferol and quercetin, respectively. The reproducibility based on migration time and peak height were 0.47% and1.87% for kaempferol, 0.55% and 2.02% for quercetin. The detection limits based on three times noise were 0.010 mg/mL and 0.008 mg/mL for kaempferol and quercetin, respectively. The developed method was utilized to analyze real samples and running recovery experiments with satisfactory results.
Authors and Affiliations
Yuanqi Lu , Dunqing Wang , Zhihua Ning , Qingqiang Yao
Keywords
Related Articles
- EP ID EP94131
- DOI -
- Views 120
- Downloads 0
Broad spectrum anti-microbial compounds producing bacteria from coast of Qingdao bays
Anti-microbial resistance burden and hazard associated with chemical treatment of infections demanded for new anti-microbial natural products. Marine associated microorganisms are the enormous source of bioactive c...
Antimicrobial profile of some novel keto esters: Synthesis, crystal structures and structure-activity relationship studies
Rapid increase in bacterial resistance has become a major public concern by escalating alongside a lack of development of new anti-infective drugs. Novel remedies in the battle against multidrug-resistant bacterial...
Analysis on active molecules in Populus nigra wood extractives by GC-MS
Populus nigra has been introduced and largely planted in China, and the waste wood was still abandoned. Therefore, the wood extractives of Populus nigra were studied to further utilize the bio-resources. The result...
Distribution of selenium and cadmium in soil-rice system of selenium-rich area in Hainan, China
Rice, which is the staple food in East Asia, is a source of Selenium (Se) and Cadmium (Cd). The distribution of Se and Cd in soil-rice system is significant to human nutrition and public health. This study is to ex...
A novel strategy for antimicrobial agent: Role of exogenous carbon monoxide on suppressing Escherichia coli vitality and toxicity
Sepsis is a severe systemic inflammatory response mostly caused by gram-negative bacterial infections. The rates of mortality in sepsis patients remain high. To date little is known about whether exogenous carbon m...