Effect of gonadotrophins, oestradiol and insulin on cumulus expansion of Nili Ravi buffalo oocytes
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 1
Abstract
The objective of the present study was to investigate the cumulus expansions of Nili Ravi buffalo oocytes during cultured in TCM -199 supplemented with 2 µg/ml oestradiol (E2), 0.05 IU/ml recombinant human follicle stimulating hormone (rhFSH), 2IU/ml human chorionic gonadotrophin (hCG), and 0.12 IU/ml insulin (I). The cumulus oocytes complexes (COCs) were collected from 2-8mm follicles from local abattoir ovaries. Supplementation of medium with single hormones showed significant (P<0.0001) increase in mean diameter of COCs with rhFSH except E2, hCG and insulin after 24 hours compared to the increase in the mean diameter of COCs matured in TCM-199 without any hormonal supplementation. With rhFSH even at 8th hour, significant increase (P<0.001) in cumulus expansion was observed. In combination of hormones the significant (P<0.0001) cumulus expansion was achieved in E2+rhFSH treatment group. The non significant (P>0.05) cumulus expansion was observed in treatment groups viz. E2+hCG, E2+Insulin, rhFSH+hCG, rhFSH+Insulin, hCG+Insulin, E2+rhFSH+hCG and E2+rhFSH+hCG+Insulin after 24 hours. In conclusion, supplementation of rhFSH alone and in combination with E2in TCM-199 has highly significant effect on cumulus expansion.
Authors and Affiliations
Beenish Shahid , Samina Jalali , Muhamad Ijaz Khan , SA Shami
Keywords
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