Efficacy of grape seed and skin extract against doxorubicin-induced oxidative stress in rat liver
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 6
Abstract
Doxorubicin (Dox) is an anthracycline used in chemotherapy, although it causes toxicity and oxidative stress. Grape seed and skin extract (GSSE) is a mixture of polyphenolic compounds with antioxidant properties. To evaluate the hepato-toxicity of Dox on healthy rats as well as the protective effect of GSSE, rats were treated with GSSE (500mg/kg bw) during 8 days. At the 4th day of treatment, they received a single dose of Dox (20 mg/kg bw). After the treatment (9th day), livers were collected and processed for oxidative stress status. Dox increased MDA (+ 900%), decreased catalase (-60%) and increased peroxidase (+90%) and superoxide dismutase (+100%) activities. In this latter case Dox mainly increased the iron isoform. Furthermore Dox altered intracellular mediators as catalytic free iron (-75%), H2O2 (-75%) and calcium (+30%). Dox also affected liver function by elevating plasma triacylglycerol and transaminases and liver morphology by altering its typical architecture. Importantly all Dox-induced liver disturbances were alleviated upon GSSE treatment. Dox induced liver toxicity and an oxidative stress mainly characterized by increased lipoperoxidation but not protein carbonylation. GSSE efficiently protected the liver from Dox-induced toxicity and appeared as a safe adjuvant that could be incorporated into chemotherapy protocols.
Authors and Affiliations
Meherzia Mokni , Sonia Hamlaoui , Safouen Kadri , Ferid Limam , Mohamed Amri , Lamjed Marzouki , Ezzedine Aouani
Keywords
Related Articles
- EP ID EP95744
- DOI -
- Views 100
- Downloads 0
High-level expression of fusion peptide Dybowskin-2CDYa and hEGF in E. coli and its dual function
Dybowskin-2CDYa (Dy2), with a broad antimicrobial spectrum and low hemolytic feature, is a newly discovered type of antimicrobial peptides from Rana dybowskii. In order to get a dual function peptide which inhibits...
Mercury exposure of gold mining workers in the northwest of Iran
Mercury exposure is a health concern in the occupational settings like gold mining and chloralkali industries and blood and urine levels of mercury are used as exposure indicators. In this study, blood and urine co...
Densitometric HPTLC method for qualitative, quantitative analysis and stability study of coenzyme Q10 in Pharmaceutical formulations utilizing normal and reversed-phase silica gel plates
Two simple, preciseand stability-indicating densitometric HPTLC method were developed and validated for qualitative and quantitative analysis of Coenzyme Q10in pharmaceutical formulations using normal-phase (Method...
Synthesis, spectral characterization and enzyme inhibition studies of different chlorinated sulfonamides
Sulfonamides are adherent to a biologically dynamic category of compounds and are under consideration of many organic synthetic researches to synthesize pharmacologically important compounds. In this demonstrated r...
Annona squamosa Linn: Cytotoxic activity found in leaf extract against human tumor cell lines
Cancer is a common cause of death in human populations. Surgery, chemotherapy and radiotherapy still remain the corner stone of treatment. However, herbal medicines are gaining popularity on account of their lesser...