In vitro studies of Aryloxyphenoxy-propionate herbicide susceptible and resistant little seed canary grass and Wheat
Journal Title: Scientia Agriculturae - Year 2015, Vol 11, Issue 2
Abstract
Little seed canary grass (Phalaris minor Retz.) is a major weed in wheat fields of Haryana, and has developed resistance to fenoxaprop and clodinafop in large area. In view of this, laboratory trials were conducted to evaluate the response of etiolated and non-etiolated seedlings of five P. minor populations along with wheat against fenoxaprop-p-ethyl, clodinafop-propargyl and two new herbicides pinoxaden and ready mix formulation of sulfosulfuron plus metsulfuron. The experiment was comprised of six treatments with each herbicide. The observations were made on different physiological parameters i.e. photosynthetic pigment development and membrane injury, and biochemical parameters i.e. lipid peroxidation and proline content. With increasing the concentration of herbicide a differential decline in total chlorophyll content was observed in all P. minor biotypes. Herbicide doses causes the increased in lipid peroxidation end product malondialdehyde, which results maximum ion efflux in HAU, Ambala and Uchana populations and marginal increase was in Chanarthal population, while in case of wheat the increase was negligible. Proline content also increased with increase herbicide concentration in all populations. Out of five studied P. minor populations, one was identified as insensitive, two were moderate sensitive and two were sensitive to fenoxaprop and clodinafop herbicides. For pinoxaden, four populations were observed as sensitive and one was insensitive, while, all P. minor populations were susceptible to ready mix formulation of sulfosulfuron plus metsulfuron. However, in wheat, no toxic effects were observed even at higher dose of all four herbicides. Based on these studies there is an opportunity to assess herbicide resistant little seed canary grass biotypes by select¬ing from a wide range of herbicides.
Authors and Affiliations
P Bhasker
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