Induction of heme oxygenase-1 attenuates chemotherapy-induced pulmonary toxicity in rats: A possible link between heme oxygenase-1 and NF-κB
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2016, Vol 29, Issue 2
Abstract
A critical restriction in the use of bleomycin (BLM) is development of pulmonary fibrosis via oxidative and inflammatory mechanisms. Drugs that induce heme oxygenase-1 (HO-1) like hemin (HEM), have anti-inflammatory, antioxidant, and immunomodulatory effects. Accordingly, it is worth to test HEM against BLM-induced lung Injury. Four groups of rats were used: control group; HEM group (50mg/kg, i.p.); BLM group (5mg/kg, intratracheal single injection) and HEM+BLM group (HEM administered 1 day before BLM injection and continued for 14 days). At the end of experiment, lactate dehydrogenase (LDH) and NO levels were estimated in bronchoalveolar lavage fluid (BALF). Hydroxyproline (HP), myeloperoxidase (MPO), IL-6, GSH, MDA levels and SOD activity were determined in lung tissues. In addition, expression of HO-1 and NF-κB protein in lung tissues was determined using both western blot and immunohistochemical techniques. Also lung tissues were investigated histopathologically. BLM produced lung damage as indicated from the elevation in LDH and NO, perturbation in lung oxidative stress indicators, increased HP, MPO, IL-6 contents and NF-κB expression. On the other side, HEM, reduced BLM harmful effects as noticed from amelioration of biochemical markers and histopathological lesions, which is concomitant with over-expression of HO-1. Therefore, induction of HO-1 in lung by HEM may alleviate the lung damaging effects of BLM.
Authors and Affiliations
Ihab Talat Abdel-Raheem , Gamal Abd El-Hay Omran
Keywords
Related Articles
- EP ID EP90811
- DOI -
- Views 82
- Downloads 0
Rapid separation and determination of betulinic acid from a complex matrix using combination of TLC and RP-HPLC
Hitherto, only a few studies are reported about using the combination of TLC and RP-HPLC for the separation and determination of analyte(s) from a complex matrix. The present study is aimed to develop a simple and rapid...
Distribution and drug resistance profile of methicillin-resistant Staphylococcus aureus after orthopaedic surgery
This paper is aimed to comprehend clinical distribution and drug-resistance situation of methicillin-resistant Staphylococcus aureus. This study applied automatic microbe instrument Microscan W /A 96 for strain ide...
The clinical diagnosis and treatment about 22 cases of limbic encephalitis were retrospectively analyzed
To summarize and analyze the clinical characteristics and treatment of limbic encephalitis, in order to provide the basis for clinical work. We retrospectively analyzed the clinical characteristics, magnetic resona...
Lipid lowering effect of Cinnamomum zeylanicum in hyperlipidaemic albino rabbits
The purpose of the present study was to investigate the lipid lowering effect of Cinnamomum zeylanicum (Cinnamon) in hyperlipidaemic albino rabbits. For this purpose, forty eight albino rabbits were randomly divided into...
The detection of YMDD mutation of hepatitis B virus by allele-specific PCR and a cross-contamination proofed device
Lamivudine (LAM) is commonly used to treat Hepatitis B virus (HBV) infection, but its use frequently induces drug resistance. Therefore, rapid and correct detection of drug-resistant HBV is important for effective...