Leuconostoc mesenteroides NRRL B-1149 as probiotic and its dextran with anticancer properties
Journal Title: Journal of BioScience and Biotechnology - Year 2014, Vol 3, Issue 1
Abstract
Leuconostoc mesenteroides NRRL B-1149 was evaluated for its probiotic properties. It displayed in vitro cell surface traits in terms of hydrophobicity, autoaggregation property and cell adhesive capacity on HeLa, murine macrophage and HT29 cell lines, which revealed its capability to adhere and colonize the intestine. The tolerance to various biological barriers such as lysozyme (100 μg/ml), gastric juice (pH 3.0) and bile salts (0.5%, w/v) with 92%, 61.6% and 219% growth, respectively, confirmed its ability to survive in extreme conditions of digestive tract. It showed bile salts hydrolase activity signifying its ability to deconjugate bile salts. The whole cells of Leuconostoc mesenteroides B-1149 showed β-galactosidase activity with 94 Miller units displaying its importance in lactose utilization. The effect of dextran from Leuconostoc mesenteroides B-1149 having α-(1,6) linear glycosidic linkages and α-(1,3) branching was evaluated against cervical cancer (HeLa) and colon cancer (HT29) cell lines. The dextran from L. mesenteroides B-1149 (100 μg/ml) showed 25% inhibition of HeLa cell lines and 1000 μg/ml dextran displayed 40% inhibition of HT29 cell lines. The effect of dextran on murine monocyte-macrophage line (J774A.1) showed increase in growth of macrophages confirming its biocompatible nature.
Authors and Affiliations
Rishikesh Shukla, Ilia Iliev, Arun Goyal
Keywords
Related Articles
- EP ID EP136507
- DOI -
- Views 87
- Downloads 0
Recent advances in Broomrapes research (ERRATUM)
Genetic and phenotypic diversity of naturally isolated wild strains of Aspergillus niger with hyper glucose oxidase production
Glucose oxidase (GOx) is the basic stone for many of biological industry worldwide. The improvement of GOx production basically depends on selection of hyper producer strain of Aspergillus niger. Selective isolatio...
A novel neutral protease from thermophilic Bacillus strain HUTBS62
A novel neutral highly thermostable protease was detected in the culture medium of thermophilic Bacillus strain HUTBS62 isolated from hot-spring located near to the Dead Sea, Jordan. The enzyme was purified by precipitat...
New data about some rare and less known discomycetous fungi from Bulgaria
New data for six discomycetes from orders Helotiales and Rhytismatales in Bulgaria are presented herein. Three of them (Ombrophila violacea, Rutstroemia bulgarioides and R. calopus) are of high conservation value.
Isolation and screening of biopolyester (poly-β-hydroxybutyrate) producing bacteria from compost samples in Bangladesh
Poly-β-hydroxybutyrate (PHB) is the simplest member of polyhydroxyalkanoates (PHAs) that are biological polyesters or biopolymers produced by a wide variety of bacteria as an intracellular storage material of carbon and...