RESISTIN AND HIGH GLUCOSE CONCENTRATIONS-ACTIVATION OF HUMAN SMOOTH MUSCLE CELLS INDUCES ENHANCED MONOCYTE CHEMOTAXIS
Journal Title: Romanian Journal of Diabetes Nutrition and Metabolic Diseases - Year 2012, Vol 19, Issue 1
Abstract
Objectives. Recent data indicate that upon activation by resistin and high glucose concentrations (HG) vascular smooth muscle cells (SMC) acquire pro-inflammatory properties. We questioned whether resistin and HG-activated SMC generate an enhanced monocytes chemotaxis and if the chemokine fractalkine (Fk) is involved in the process. Material and Methods: SMC were incubated with resistin or/and HG and the conditioned medium was used for monocytes chemotaxis assays. The role of Fk was assessed by blocking the Fk receptor, CX3CR1, on monocytes (U937 cell lines) prior to the chemotaxis assay. The quantification of migrated monocytes was assayed under an inverted microscope and statistically analyzed. Results: (i) conditioned medium (CM) collected from SMC incubated with resistin in the presence or absence of HG triggered a significant increase (25 – 100 %) of monocytes chemotaxis as compared to controls; (ii) blocking the CX3CR1 receptor significantly decreased the monocyte chemotaxis towards resistin–treated SMC. Conclusions: Resistin±HG increases the expression of chemotaxis inductors in human SMC and the ensuing monocytes chemotaxis by a mechanism in which Fk plays a major role.
Authors and Affiliations
Viorel Simion, Ana-Maria Gan, Daniela Stan, Monica Pirvulescu, Manuela Calin , Elena Butoi , Ileana Manduteanu
Keywords
Related Articles
- EP ID EP131447
- DOI 10.2478/v10255-012-0003-7
- Views 87
- Downloads 0
NC Paulescu’s “Traite de Physiologie Medicale”
METABOLIC SYNDROME IN PATIENTS WITH T1DM OLDER THAN 10 YEARS
Metabolic Syndrome (MS) is a clinical entity recently individualized, a clinical concept with multiple definitions that expresses a complex disorder of the body′s energy metabolism, with insulin resistance and compensato...
HIGH PREVALENCE OF METABOLIC DISORDERS IN PSORIASIS PATIENTS
Recent studies have shown that psoriasis is a systemic disorder associated with an increased prevalence of metabolic anomalies. This study compares the prevalence of obesity and metabolic disorders in psoriasis patients...
Glucose Variability In Persons With Diabetes Evaluated By Continuous Glucose Monitoring
Blood glucose level is the main indicator of diabetes control. It is related to diabetes evolution and appearance of chronic complications. Daily and/or postprandial glucose fluctuations induce oxidative stress and endot...
THERAPY WITH GLP-1 AGONISTS AND DIPEPTIDYL-PEPTIDASE IV INHIBITORS IN TYPE 2 DIABETES MELLITUS
Since type 2 diabetes is increasing and most patients do not reach their therapeutic goals, novel treatment options are needed. The novel therapeutic options for type 2 diabetes mellitus (Type 2 DM); respectively those b...