SEROLOGICAL PREVALENCE OF ANTI-TOXOPLASMA ANTIBODIES AMONG IMMUNOCOMPETENT POPULATION -COMPARATIVE STUDY OF ELISA AND LATEX AGGLUTINATION TEST
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2013, Vol 2, Issue 23
Abstract
Toxoplasma gondii (T gondii) causing toxoplasmosis is highly prevalent throughout the world. Transfusion or organ transplantation from an infected person can transmit the infection. Studies performed in different geographic areas and populations have shown need for regional studies to determine epidemiologic and socio-demographic characteristics for each population in order to improve control and prevention strategies. Seroprevalence can be associated with environmental and cultural factors. Different methods like Latex Agglutination Test (LAT) and Enzyme Linked Immuno Sorbent Assay (ELISA) are available for the detection of antitoxoplasma antibody. The study was planned to conduct a comparative evaluation of ELISA and LAT techniques for the serological prevalence of anti-toxoplasma antibodies among immunocompetent population, in the department of Neuromicrobiology, National Institute of Mental Health and Neurosciences (NIMHANS) Bengaluru. As a prospective study it comprised a total of ninety serum samples collected from the healthy voluntary blood donors attending Transfusion medicine centre. Serum was tested for T. gondii antibodies by LAT and ELISA. The age group ranged from 18 to 45 years. All the serum samples were seronegative for Human Immunodeficiency Virus (HIV) and clinically there was no other evidence ofToxoplasma gondii (T gondii) causing toxoplasmosis is highly prevalent throughout the world. Transfusion or organ transplantation from an infected person can transmit the infection. Studies performed in different geographic areas and populations have shown need for regional studies to determine epidemiologic and socio-demographic characteristics for each population in order to improve control and prevention strategies. Seroprevalence can be associated with environmental and cultural factors. Different methods like Latex Agglutination Test (LAT) and Enzyme Linked Immuno Sorbent Assay (ELISA) are available for the detection of antitoxoplasma antibody. The study was planned to conduct a comparative evaluation of ELISA and LAT techniques for the serological prevalence of anti-toxoplasma antibodies among immunocompetent population, in the department of Neuromicrobiology, National Institute of Mental Health and Neurosciences (NIMHANS) Bengaluru. As a prospective study it comprised a total of ninety serum samples collected from the healthy voluntary blood donors attending Transfusion medicine centre. Serum was tested for T. gondii antibodies by LAT and ELISA. The age group ranged from 18 to 45 years. All the serum samples were seronegative for Human Immunodeficiency Virus (HIV) and clinically there was no other evidence of
Authors and Affiliations
Nagarathna S, Veenakumari HB, Cynthia Kasi, Sunder P
Keywords
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- EP ID EP158989
- DOI 10.14260/jemds/811
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