SIMULTANEOUS QUANTIFICATION OF EPIGALLOCATECHIN-3-GALLATE AND ATORVASTATIN BY USING HPLC-UV METHOD AND ITS APPLICATION TO PHARMACOKINETIC STUDIES IN RATS
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 5
Abstract
ABSTRACT A sensitive, precise and simple LC method for the simultaneous quantification of epigallocatechin-3-gallate and atorvastatin in rat plasma has been developed and validated. The chromatographic separation was achieved on a C18 column (250mm×4.6 mm, 5 μm) maintained at room temperature, using gradient elution with 0.1% formic acid (Solvent A) and acetonitrile (Solvent B), and detected using UV-visible detector. Protein precipitation followed by liquid-liquid extraction of epigallocatechin-3-gallate and atorvastatin from rat plasma resulted in their high recoveries. LC calibration curves based on the extracts from rat plasma were linear in the range of 30–1000 ng/ml for both the analytes. The limits of quantification were 30ng/ml for both epigallocatechin-3-gallate as well as atorvastatin. The precision and accuracy of the method were well within the generally accepted criteria for biomedical analysis. The described method was successfully applied to study the effect of epigallocatechin-3-gallate, which is reported as a P-glycoprotein inhibitor on the pharmacokinetics of atorvastatin (P-glycoprotein substrate) in Wistar rats. The results of the study inferred that epigallocatechin-3-gallate significantly improved the oral bioavailability of atorvastatin. Keywords: Column liquid chromatography,P-glycoprotein, Epigallocatechin-3-gallate, Atorvastatin, Pharmacokinetics.
Authors and Affiliations
Ranjeet Prasad Dash and Manish Nivsarkar*
Keywords
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