STUDIES REGARDING IN VITRO REGENERATION OF ARNICA MONTANA L. FROM NATURAL POPULATIONS - BISTRITA VALLEY (EASTERN CARPATHIANS)
Journal Title: Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi, Serie noua, Sectiunea II a. Biologie vegetala - Year 2010, Vol 56, Issue 1
Abstract
Arnica montana, is a rare and vulnerable species, traditionally used for medicinal purposes. Our approach aims at the possibility of using in vitro multiplication as a complementary component for the initiation and development of the species conservation strategy in the mountain valley of Bistrita in the Eastern Carpathians. For in vitro regeneration, the biological material used to initiate tissue cultures, comes from Arnica montana seedlings, resulting from germination of seeds collected from natural populations. On Murashige and Skoog (1962) solidified multiplication medium, supplemented with different amounts of BAP* and NAA**, we obtained a total of 2-3 neoplantlets/explant. Bud multiplication was succeeded by rooting phase, the neoplantlets being transferred for ex vitro acclimatization, with a survival rate of 75-80% after invigorate plants are transplanted in situ. Preliminary phytochemical analysis carried out by TLC and HPLC-UV in plants from natural populations of A. montana showed the presence of compounds of the polyphenolic and flavonoidic types.
Authors and Affiliations
CAMELIA STEFANACHE, DOINA DANIL, A. NECULA, ELVIRA GILLE
Keywords
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