The establish of the HPLC method to examine the plasma concentration of lamotrigine and oxcarbazepine
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2015, Vol 28, Issue 3
Abstract
To establish the HPLC method to examine plasma concentration of lamotrigine and oxcarbazepine. This study set chlorzoxazone as the internal standard, chromatographic column was Column C18 (200×4.6mm, 5um) of DIKMA company, the mobile phase was methanol, water and trifluoroacetic acid, with rate of 40: 60: 0.0005, at a flow rate of 1 mllmin-1, the detected wavelength was 240 nm. The plasma concentrations of lamotrigine was 0.5-50ug•mL-1, the standard curve was excellent for Y=0.5511C-0.5669, r=0.9940, average recovery was 91.40%; The plasma concentrations of oxcarbazepine was 0.5-50ugmL-1, the standard curve was good for Y=0.4026C-0.5895, r=0.9925, and the average recovery was 89.59%; The three plasma concentrations of lamotrigine were respectively 25μg•mL-1, 10 μg•mL-1 and 2μg•mL-1 and its five parallel sample for injection RSD were respectively 4.01%, 6.15% and 4.64%; The three plasma concentration of oxcarbazepine were 25μg•mL-1, 10μg•mL-1 and 2μg•mL-1, and its five parallel sample for injection RSD were respectively 3.05%, 4.27% and 9.01%. This method was easy to operate, high recovery and high precision, and was applicable to the clinical detection for plasma concentration of lamotrigine and oxcarbazepine.
Authors and Affiliations
Xue-Ping Zhu , Yao-Dong Zhao , Zhi Cheng , Ning Min Zhao , Hao Li
Keywords
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