Nephroprotective Effect of Ethanolic Extract of Flax Seed
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 7
Abstract
ABSTRACT Introduction: The treatment of Acute Kidney Injury (AKI) remains empirical and hence the quest for an effective agent for this condition still remains a challenge. The nephroprotective effect of flax seeds has been proven in pre-clinical models of kidney injury like lupus nephritis and in model of Type 2 diabetes mellitus where flax seeds meal ameliorated proteinuria. Aim: To evaluate the nephroprotective effect of Ethanolic Extract of flax seed/Linum Usitatissimum (EELU) in Gentamycin induced nephrotoxicity in Wistar rats. Materials and Methods: The study was initiated after Institutional Animal Ethics Committee approval. A total of 46 adult male Wistar rats were randomly divided into six groups with eight rats in each group except normal control (n=6) (Group I). The positive control (Group III) received alpha lipoic acid (25 mg/kg/day p.o). The three study groups received EELU in dose 0.7 (Group IV), 1.4 (Group V) and 2.8 (Group VI) mg/kg/day per oral (p.o.) respectively. All groups, except normal control group (Group I), received gentamycin 150 mg/kg/day intraperitoneal (i.p.) for 10 days. The nephroprotective effect of EELU was assessed using following variables body weight, Blood Urea Nitrogen (BUN), Serum creatinine, kidney Malondialdehyde (MDA), and Glutathione (GSH) levels and kidney injury on histopathology. One-way ANOVA was used to analyse parametric data while the non-parametric data was analysed using Kruskal Walis test. Results: EELU in the dose of 2.8 mg/kg/day showed significant improvement in BUN (27.89±6.44 mg/dL), serum creatinine (1.3±0.29 mg/dL), MDA (196.88±17.88 mmol/gm), GSH (1.63±0.12 μgm/gm) and histopathology as compared to Disease control (p>0.05). These findings were statistically similar to positive control group (p<0.05). EELU in the dose of 1.4 mg/kg/day showed significant improvement in BUN (29.34±7.30 mg/dL), serum creatinine (1.89±0.26 mg/dL), MDA (235.71±22.42 nmmol/gm), GSH (1.37±0.22 μgm/gm) and histopathology as compared to Disease control (p>0.05). Improvement in BUN and histopathology was statistically similar to positive control group (p<0.05). EELU in the dose of 0.7 mg/kg/day showed significant improvement in BUN (34.24±3.10 mg/dL), serum creatinine (1.91±0.67 mg/dL), MDA (261.86±23.22 nmmol/gm) as compared to Disease control (p>0.05). Conclusion: The present study demonstrated the nephroprotective effect of EELU in high dose (2.8 mg/kg/day) in the model of gentamycin-induced nephrotoxicity in Wistar rats and prevented the acute kidney injury most probably due to its antioxidative potential. Findings of the study suggest that EELU can be used as therapeutic agent in patients who are at risk of kidney injury and further clinical studies should be planned.
Authors and Affiliations
Shruti Bhide, Sunil Bhojne, Hemant Kanase, Sanket Raut, Pradnya Dethe
Keywords
Related Articles
- EP ID EP606820
- DOI 10.7860/JCDR/2019/41506.13025
- Views 135
- Downloads 0
Comparison of Cone Beam Computed Tomography-Derived Alveolar Bone Density Between Subjects with and without Aggressive Periodontitis
Introduction: Understanding the changes in bone density of patients affected by aggressive periodontitis could be useful in early disease detection and proper treatment planning. Aim: The aim of this study was to compare...
The Relationship between Location and Size of Nephrolith in the Pelvicalyceal System
ABSTRACT Introduction: Nephrolithiasis is a common health problem worldwide. Both location and size of nephrolith are essential factors in planning of clinical management. Due to lack of previous studies on the relation...
A Clinical Study of Colour Vision Status of Female Relatives of Indian Males with Defective Colour Vision
ABSTRACT Introduction: Around 15% of females are carriers of congenital colour blindness. Variable colour vision status exists in these heterozygous carrier females due to various factors such as retinal mosaicism and th...
Application of a Mixed Material Graft to Posterior Intravaginal Slingoplasty Procedure: Preliminary Pilot Series
Good anatomic outcomes have been achieved with transvaginal synthetic materials in pelvic organ prolapse, but mesh-related complications are a major health concern. Therefore, developing new synthetic or biological mater...
Shear Bond Strength of Metal Brackets to Zirconia Following Different Surface Treatments using a Universal Adhesive
ABSTRACT Introduction: As there is increase in demand of orthodontic treatment by adult patients, bonding of orthodontic brackets to dental restoration surfaces is now a common necessity because most adult patients have...