Abstract

Objective: To establish the protective effect of seaweed Colpomenia sinuosa against carbon tetra chloride (CCl4) induced oxidative stress and resultant dysfunction of rat kidney. Methods: Seven to eight weeks old male Wistar rats (150-220g) were exposed to CCl4 (1.5 ml/kg) injection then treated with seaweed Colpomenia sinuosa (100 mg/kg body weight in 0.3% CMC solution). Blood was collected at the 5th day of experimental period to estimate the Total count (TC), Hemoglobin (HB), Total protein (TP), Glucose, Albumin, Cholesterol, TGL and Urea. Results: The results shows significantly decreased (P<0.01) level of TC, the cholesterol and urea levels shows significantly increased (P<0.05) in CCl4 treated groups when compared to control groups. These levels were found to be normalized by oral feeding of C. sinuosa. Then the rats were sacrificed and kidneys taken for enzyme analyses and histological examination. In the CCl4 treated group significantly increased activities in TBARS, SOD, CAT, GPX, GSH (P<0.05) when compared to control group. These increased activities were found to near normal in the CCl 4 + C. sinuosa treated group and Seaweed C. sinuosa treated alone group did not change any enzyme activity. Exposure to CCl4 resulted hydrobhic changes in epithelium and Hypercellulartity of glomerulus was seen in the CCl 4 + drug treated group. Conclusions: These results suggest that the nephroprotective effect of C. sinuosa can be attributed to its enhancing effects on antioxidant defense system and lead to prevent the damage by exposure of CCl4 toxicity.

Authors and Affiliations