Nocardiopsis sp. 5 Endophytic to Tulsi Leaves – Isolation and Antimicrobial Activity
Journal Title: Microbiology Research Journal International - Year 2015, Vol 5, Issue 3
Abstract
Aims: To isolate and identify the endophytic Nocardiopsis sp.5 from Tulsi (Ocimum sanctum) leaves and estimates its antimicrobial activity against various human pathogens and tea pathogens. Study Design: Rare endophytic actinobacteria isolation, identification and estimation of antimicrobial activity. Place and Duration of Study: Department of Botany and Department of Bioinformatics, Nirmala College for Women, Coimbatore – between December 2011 and December 2012. Methodology: Pre-sterilization of Ocimum sanctum leaves and isolation of rare endophytic actinomycetes using selective media. Estimation of antimicrobial activity of these isolates by primary and secondary streaking methods. Morphological and Molecular Identification of the most bioactive isolate. Results: Out of 11 endophytes, four isolates (2, 3, 5, and 9) showed antagonistic activity against tea and human pathogens. Among these four isolates, isolate no.5 showed maximum activity against all the human pathogenic microorganisms such as Staphylococcus aureus (21mm), Candida albicans (18 mm), Enterococcus faecalis (19mm), Vibrio cholera (18mm) and tea pathogens such as Glomerella cingulata (14mm) Hypoxylon serpens (17mm) and Pestalopsis theae (18mm). Hence, morphological and phylogenetic studies show that isolate no.5 belongs to Nocardiopsis group, showing 99% similarity to both Nocardiopsis synnemataformans and Nocardiopsis dassonvillei. Conclusion: Endophytic Nocardiopsis strain 5 was shown to produce good antimicrobial activity against plant pathogens and human pathogens. Further purification, structure elucidation and characterization of the antimicrobial compound from Nocardiopsis 5 strain are recommended. There is definite scope for bioprospecting of antagonistic actinomycetes from Western Ghats once appropriate consistent studies are undertaken.
Authors and Affiliations
Mrinalini J. Singh, S. Padmavathy
Keywords
Related Articles
- EP ID EP354679
- DOI 10.9734/BMRJ/2015/13523
- Views 85
- Downloads 0
Impact of Mass Praziquantel Administration for Controlling Schistosoma haematobium Infection in Schoolchildren from Bamako, Mali
Background: Uro-genital schistosomiasis caused by Schistosoma haematobium is prevalent in sub-Saharan Africa. For the control of the disease, the frequent and periodic use of mass praziquantel administration (MPA) is rec...
Tolerance to Heavy Metals by Some Fungal Isolates from Petroleum Refinery Effluent in Kaduna, Nigeria
Investigation was carried out to assess the potential of some fungal species to tolerate and grow in biomass in vitro in the presence of some heavy metals (HMs) (Pb, Zn, Cr and Cd) from Kaduna Refinery and Petrochemical...
In silico RFLP Analysis of 16S rRNA Genes: A Helpful Application for Distinguishing Bifidobacteria from Human and Animal Source
Bifidobacterial species are widespread in gastrointestinal tracts of mammalian and other animals; they can be found in extra body environment only after a fecal contamination or human intentional addition (as the case of...
Marine Environment: A Potential Pool of Isolating Antibiotic Producing Bacteria with Novel Characteristics
Aims: This review article analyze the data based on antibiotic producing bacteria isolated from different poles of marine environment globally and further more their antibiotic potential against different pathogenic micr...
Molecular Identification and Characterization of Heavy Metal Resistant Bacteria and Their Role in Bioremediation of Chromium
In this study two bacterial strains Bacillus thuringiensis (Cr-S1) and Bacillus pumilus (Cr-S2) were isolated on Cr+6 enriched nutrient agar plates at the concentration of 100 µg/ml. Minimum inhibitory concentration of c...