Non rhizobial endophytic bacteria from chickpea (Cicer arietinum L.) tissues and their antagonistic traits
Journal Title: Journal of Applied and Natural Science - Year 2019, Vol 11, Issue 2
Abstract
Bacteria that colonize plant tissues other than rhizobia and are beneficial for plant growth referred to non rhizobial plant growth-promoting endophytic bacteria (PGPEB). This study was designed to assay the biocontrol activity of plant growth promoting endophytic bacterial isolates those found positive for P. solubilization, ACC deaminase, Indole acetic acid and Gibberelic acid production. These bacterial isolates were obtained from chickpea (Cicer arietinum L.) tissues (roots and nodules). In a previous study a total of 263 non rhizobial endophytic bacterial isolates were isolated. Out of 263 isolates, 64.5% and 34.5% were Gram positive and negative, respectively. Further for biochemical characterization, catalase, oxidase, citrate utilization, nitrate reduction, methyl red and Voges Proskauer’s tests, were performed. On the basis of P solubilization, ACC deaminase, Indole acetic acid and Gibberelic acid production 75 potential isolates were selected and screened for their biocontrol activity viz. (production of cell wall degrading enzymes, production of HCN and fluorescent pigment). Out of 75 isolates, only 29 isolates produced cellulase, 64 isolates were able to produce protease and 28 were positive for both cellulose and protease. Of 75 endophytic isolates 12 isolates (7 from root tissue and 5 from nodules tissue, respectively) were positive for HCN production and 16 isolates were found to be fluorescent pigment producer under µv ligh. As chemical fertilizers and pesticides have detrimental effects on the environment. So these bacterial endophytic isolates will be used not only as a biofertilizer because of their plant growth promotional activities but also used as an alternative of synthetic chemicals for control of several plant diseases.
Authors and Affiliations
Deepika Chhabra, Poonam Sharma
Keywords
Related Articles
- EP ID EP588668
- DOI 10.31018/jans.v11i2.2056
- Views 69
- Downloads 0
Effect of feed supplemented exogenous bacteria, Lactobacillus sporogenes on the growth and body composition of Cirrhinus mrigala fingerlings
Probiotics are known to improve the growth rate of aquatic organisms. Little experimentation has focused on supplementation of live colony forming units ofL.sporogenes in Cirrhinus mrigala to access the growth performanc...
Demography of helminth parasites in relation to biometic characteristics of Mastacembalus armatus
The study was conducted to collect and identify helminth parasites of Mastacembalus armatus and also to determine the prevalence intensity and abundance of parasitic infection. 118 M. armatus, obtained from different sa...
A study on geospatial technology for detecting and mapping of Solenopsis mealybug (Hemiptera: Pseudococcidae) in cotton crop
Detection of crop stress is one of the major applications of remote sensing in agriculture. Many researchers have confirmed the ability of remote sensing techniques for detection of pest/disease on cotton. The objective...
Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens
Isolation of endophytic actinomycetes is an important step to screen antimicrobial compounds to curb the threat of drug-resistant strains of human pathogens. Out of the 50 endophytic actinomycetes obtained from surface s...
Contamination of Asian herbal drugs: Need for its critical evaluation
Herbal drugs have been used as remedies for the treatment of large number of humans, ailments since ancient times as the traditional medicine system; India has a glorious past, having first documented record of fully dev...