NOVEL RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF METFORMIN AND PIOGLITAZONE DRUGS IN PURE AND PHARMACEUTICAL DOSAGE FORMS
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 5
Abstract
The present investigation describes about a simple, economic, selective, accurate, precise reverse phase high performance liquid chromatographic method for the simultaneous estimation of metformin and pioglitazone in pure and pharmaceutical dosage forms. Metformin and pioglitazone were well separated using a HYPERSIL BDS C18column of dimension 250 × 4.6, 5µm and Mobile phase consisting of Phosphate buffer( Pottasium dihydrogen phosphate) : Methanol (Adjusted with Ortho phosphoric acid to pH-4.5) in the ratio of 70:30v/v at the flow rate 1 ml/min and the detection was carried out at 240nm with PDA detector. The Retention time for Metformin and Pioglitazone were found to be 1.945, 3.595min respectively. The developed method was validated for recovery, specificity, precision, accuracy, linearity according to ICH guidelines. The method was successfully applied to Metformin, and Pioglitazone combination pharmaceutical dosage form.
Authors and Affiliations
Alekhya Pallapolu, Aneesha A
Keywords
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- EP ID EP33118
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