Observations of Enamel Microstructure in Incipient Lesions Remineralized by NaF Dentifrices
Journal Title: Dental Research and Management - Year 2017, Vol 2, Issue 1
Abstract
Synchrotron Radiation (SR) Wide-angle X-ray Diffraction (WAXD) and Small-angle X-ray Scattering (SAXS) techniques were used to assess microstructure of bovine enamel white-spot lesions (WSL) evaluated in a 10-day pH cycling model comprising three different dentifrice groups: (A) 0.21% NaF plus TCP (Clinpro Tooth Crème), (B) 1.1% NaF plus TCP (Clinpro 5000), or (C) 0% NaF (Tom’s of Maine) dentifrice. Each day consisted of four 2-minute treatments, one 4-hour acid challenge (pH=5.0), and immersion in artificial saliva (pH=7.0) between these events. These specimens were also examined with cross-sectional microhardness, digital light microscopy and FE-SEM (field emission scanning electron microscope), and demonstrated the remineralization model effected changes in subsurface microstructure. X-ray diffraction data from WAXD and SAXS were collected on enamel slab cross-sections extending from 0 μm to 150 μm, in 6 μm microbeam increments. A primary outcome of this observational study was that simultaneous WAXD and SAXS measurements were able to resolve significant differences (ANOVA, Student’s t-test, p<0.05) between the effects of the two fluoride-containing dentifrices on subsurface lesion microstructure. In particular, enamel lesions treated with 1.1% NaF dentifrice group manifested an abundance of nanometer-sized crystallites, while treatment with the 0.21% NaF dentifrice produced larger apatite-like crystals. While the presence of fluoride in both cases promoted regularity in crystal size and orientation, this was not observed for lesions treated without fluoride. Altogether, our observations demonstrate the pathological processes for remineralization are markedly influenced by the presence and concentration of fluoride, the microstructural characteristics of which can be distinguished using the simultaneous WAXD and SAXS technique.
Authors and Affiliations
Makoto Asaizumi
Keywords
Related Articles
- EP ID EP399264
- DOI -
- Views 264
- Downloads 0
Non-Albicans Candida in Oral Cancer
Mouth is an important source of infections and oral infections increase the risk of mortality. Invasive Candida infections (ICI) are associated with high mortality and non-albicans Candida are responsible for most o...
A Study of Composite Surface Hardness When Cured Using Special Accessories with a Curing Light
The study was carried out to compare the Vickers Micro-Hardness (VHN) of composite resin of different diameters (6 mm and 10 mm) prepared in a split mold. The 6 mm and 10 mm diameter composite resin samples were cured wi...
Influence of the Knowledge and Practices on Dental Students Career Choices of Specialty in Saudi Arabia
Introduction: The dental profession plays a significant role in the service of the society. The selection of dentistry as a career as well as choosing specialty and continuing Postgraduate Dental Education (PGE) is a cri...
The Detection of Pre-Diabetic Patients in the Dental Setting
Pre-diabetes is a serious health condition where blood sugar levels are higher than normal, but not high enough yet to be diagnosed as type 2 diabetes. Pre-diabetes puts one at an increased risk of developing type 2 diab...
Principles of Soft Tissue Management in Dental Implants
Objective: To evaluate the impact of soft tissue factors in dental implants. Data: Studies evaluating the role of soft tissues in dental implants were included in this review. Sources: A comprehensive literature searc...