Ocena ekspresji Foxp3 i RORgamma t w jednojądrzastych komórkach krwi obwodowej u chorych z rakiem krtani jako wskaźników zaawansowania zmian nowotworowych — badania wstępne
Journal Title: Otolaryngologia Polska - Year 2011, Vol 65, Issue 5
Abstract
The degree of activation of cells involved in cellular immune re-sponse against tumor antigens (cytotoxic lymphocytes Te) as well as efficiency of the mechanisms which promote immunosuppression (Treg — regulatory cells CD4±CD25±Foxp3±) may determine the course of the neoplastic disease. The aim of this study was to assess the function of autologous peripheral blood mononuclear cells (PBMCs) involved in the immunological processes on the basis of expression of Foxp3 and RORgamma t molecules as well as analysis of the relationships with clinical and morphological features of the tumor (pT and pN stage, G feature, degree of invasiveness according to the TFG classification) in laryngeal carcinoma. Materia! and methods: The analysis included a group of 59 patients with verified squamous celt carcinoma of the larynx. In the pathologic evaluation pTNM classification criteria, depth of invasion and degree of histological dif-ferentiation were used. Expression levels of mRNA for Foxp3 and RORgamma t in peripheral blood mononuclear cells by quantitative analysis of the amplified product in real time (real-time RT2-PCR) were evaluated. The level of Foxp3 and RORgamma t protein expression by Western blot analysis was determined. Results: In squamous celt carcinomas of the larynx, with the highest tumor aggressiveness the significantly highest level of mRNA and protein expres-sion for Foxp3 molecule were observed. The severity of Foxp3 expression at both gene and protein level were positively linearly correlated with the degree of local extent of the tumor (pT3-4), depth of invasion (invasion of cartilage) and the degree of histological differentiation (low-differentiated tumors G3). In the study group of laryngeal cancers significantly lower level of RORgamma t expression in carcinomas with less invasive changes (pT1-2, high-differentiated tumors G1, carcinomas with microinvasion without evi-dence of invasion beyond the lamina propria) was also noted. Conclusions: The study results indicate the important role of immune celt activity as indicators of advancement of clinical and morphological changes in squamous celt carcinoma of the larynx
Authors and Affiliations
Paweł Papież, Magdalena Bryś, Iwona Lewy-Trenda, Olga Stasikowska, Jan Woś, Wiesław Papierz
Keywords
Related Articles
- EP ID EP77630
- DOI -
- Views 97
- Downloads 0
Rak brodawkowaty w torbieli środkowej szyi
Authors present a rare case of the papillary thyroid carcinoma in thyroglossal duct cyst in a 40 year-old woman. Preoperative assessment (palpation and ultrasonography) showed symptoms of typical thyroglossal duct cyst a...
‘‘Starplasty’’ tracheotomy in children of Pediatric ENT Department of Poznan University of Medical Sciences
Introduction: Obvious anatomical differences between larynx and trachea of the adult and the child necessitate different surgical techniques. The ‘‘starplasty’’ technique of pediatric tracheotomy was introduced in 1998 a...
Submucosal arytenoidectomy with laterofixation in the treatment of bilateral vocal fold paralysis following lung cancer
Introduction: Submucosal arytenoidectomy is one of many surgical techniques used in the treatment of the median bilateral vocal fold paralysis. The technique evolved from the large extralaryngeal operation to the endosco...
Rak glinianki przyusznej: analiza kliniczno-patologiczna oraz ocena wyników leczenia
<a href=# onclick=document.zmienLangEN.submit()>Streszczenie dost&#281;pne w j&#281;zyku angielskim!</a>
Strategia w leczeniu operacyjnym przewlekłego zapalenia ucha środkowego
Abstract<br/><br/>