Optimal parameters in the transformation of papaya somatic embryos using a low pressure gene gun
Journal Title: Biotecnología Vegetal - Year 2002, Vol 2, Issue 2
Abstract
Genetic improvement of papaya (Carica papaya L.) is of great importance for its commercialization in the world. In this sense biolistic, as a genetic transformation method constitutes an alternative. Using a low pressure gene gun, papaya somatic embryos were bombarded with the plasmid pCAMBIA3301. The assays were evaluated by histochemical analysis of the gus gene expression. Optimal genetic transformation conditions were obtained with a maximum efficiency of 77.8 blue points per 100 mg of somatic embryos.
Authors and Affiliations
Leticia Mas, Boris Chong, Rafael Gómez-Kosky, Jorge Gallardo, Idalia Herrera, Maritza Reyes
Keywords
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