OPTIMIZATION AND VALIDATION OF THE SIMULTANEOUS DETERMINATION OF VILDAGLIPTIN AND METFORMIN HCL IN HUMAN PLASMA BY RP-HPLC METHOD
Journal Title: Journal of Pharmaceutical and Scientific Innovation - Year 2018, Vol 7, Issue 6
Abstract
A new and simple method was developed for quantitative determination of Vildagliptin (VILD) and Metformin (MET) spiked with plasma using an Onyx C18 Monolithic column connected with an Onyx C18 guard cartridgeand PDA detection at 220 nm. The mobile phase methanol (MeOH)/acetonitrile (ACN)/potassium dihydrogen orthophosphate (KH2PO4)was pumped at 0.4 mL/min. The method was linear between 0.5-2.5 µg/mL, statistically validated for its linearity, precision, and accuracy. The precision was found to be less than 1% of the assay method. It was found that the additives in the commercial tablet did not interfere with the method. The currently developed method can routinely use for the estimation of VILD and MET related compounds from the tablet dosage form spiked with plasma.
Authors and Affiliations
Balamurugan K, Kirtimaya Mishra, Suresh R
Keywords
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- EP ID EP631512
- DOI 10.7897/2277-4572.076111
- Views 75
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