Optimization of incubation temperature in embryonated chicken eggs inoculated with H9N2 vaccinal subtype of avian influenza virus
Journal Title: Veterinary Research Forum - Year 2013, Vol 4, Issue 3
Abstract
There are little information about growth properties of low pathogenic (LP) avian influenza virus (AIV) in embryonated chicken eggs (ECEs) at different incubation temperatures. Knowledge of this information increases the quantity and quality of antigen in vaccine production process. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated (Intra-allantoic) into 400, 11-day old specific pathogen free (SPF) ECEs in the 0.1 mL per ECE rate and incubated in 32, 33, 34, 35, 36, 37.5, 38, 39 ˚C for 72 hr in 65% humidity. Early death embryos in first 24 hr were removed. Amnio-allantoic fluid was withdrawn into the measuring cylinder, and tested for hemagglutination (HA) activity and egg infective dose 50 (EID50). The utilizable ECEs and amnio-allantoic fluid volume was significantly increased in 35 ˚C, (p < 0.05). Significant difference in HA and EID50 titers, were seen only in 39 ˚C group. Therefore, 35°C is an optimum temperature for incubation of inoculated ECEs.
Authors and Affiliations
Iraj Khalili , Rahim Ghadimipour, Ali Ameghi, Saeed Sedigh-Eteghad
Keywords
Related Articles
- EP ID EP161968
- DOI -
- Views 95
- Downloads 0
Closing gaps: Integrating food safety management systems into the veterinary curriculum a tool to improve food quality and trade
Effects of chicory root powder on growth performance and histomorphometry of jejunum in broiler chicks
In the present study, chicory root powder (CRP) as growth promoter at 1% and 3% levels was supplemented in broilers’ diet to investigate the growth performance and histo-morphometry of jejunum. One hundred twenty, one-da...
Calcium, Magnesium and Total Antioxidant Capacity (TAC) in Seminal Plasma of Water Buffalo (Bubalus Bubalis) Bulls and their Relationships with Semen Characteristics
In order to determine calcium (Ca), magnesium (Mg) content and total antioxidant capacity (TAC) of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected...
Effect of curcumin on rat sperm morphology after the freeze-thawing process
Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this st...
Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa)
Acacia nilotica (Thorn mimosa) is used locally for various medicinal purposes by traditionalists and herbalists in northeastern Nigeria. Plants products have been used since ancient times in the management of various con...