Optimization of Microwave-Assisted Extraction of Bioactive Compounds from Flavorings Roots of Plant Carissa edulis
Journal Title: Scholars Journal of Engineering and Technology - Year 2016, Vol 4, Issue 3
Abstract
Abstract: The present study reports the extraction of bioactive compounds from Carissa edulis roots. Composite Center Design (CCD), a widely used form of Response Surface Methodology(RSM), was used to investigate the effect of process variables on the Microwave–Assisted Extraction(MAE). Four independent variables including extraction time (s), microwave power (W), methanol concentration (%), solvent-to-material ratio (mL/g), were studied on the response (total phenolic compounds, total flavonoids, total antioxidant activity and 1,1-diphenyl-2-picrylhydrazyl assay (DPPH). Analysis of variance showed that all factors were significant at the 5% on the measured effects. The results showed that the optimal MAE condition was obtained with an extraction time of 67.75s, microwave power of 536.41W, a methanol concentration of 43.82% and a solvent-to-material ratio of 12.55ml/g for a combination of (total phenolic compounds (5482.15 µg Gallic acid equivalents/g of Matter (µg GAE/g)), total flavonoids (206.89 µg Quercetinequivalents/g of Matter (µg QE/g) total antioxidant activity (12094.5 µg equivalents of Ascorbic Acid/g of Matter (µg EAA/g) and DPPH radical scavenging (75.10%)). Close agreement between experimental and predicted values was found. This methodology could be applied in the extraction of bioactive compounds in the natural product of industry. Keywords: Carissa edulis, microwave –assisted extraction, phenolic compounds, antioxidant activities.
Authors and Affiliations
Gertrude Eléonore Tchienou Djiobie, Roli Karole Tsatsop Tsague, Kenmogne Sidonie, Martin Benoît
Keywords
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