Optimization of nutritive medium composition for production of amylase by Bacillus strains
Journal Title: Journal of BioScience and Biotechnology - Year 2018, Vol 7, Issue 2
Abstract
The current study aimed to examine the amylolytic activities of various species of the Bacillus genus. A total of 31% of the tested 166 strains showed a positive reaction on starch agar. Their amylolytic activity was in the range of 0.9 to 2.8 U/ml. Bacillus cereus №10 showed the highest initial activity, established in the late stationary phase of growth (36 hours of incubation). The effects of different nitrogen sources, metal ions and different type of secondary carbon sources (starch was used as a primary carbon source) on the production of amylase by the screened strain were studied. The enzyme production was significantly influenced by the type and concentration of the secondary carbon source. Replacement of peptone by yeast extract and the addition of 1 mM Ca2+ increased initial amylolytic activity by 153% reaching 4.29±0.63 U/ml.
Authors and Affiliations
Yordan Stefanov, Ivan Iliev, Mariana Marhova, Sonya Kostadinova
Keywords
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