OPTIMIZATION OF THE NUTRIENT MEDIUM FOR CULTIVATION OF PSEUDOMONAS MALTOPHILIA ONU329 STRAIN – SORBENT OF HEAVY METALS IONS AND DESTRUCTOR OF OIL HYDROCARBONS
Journal Title: Мікробіологія і біотехнологія - Year 2016, Vol 0, Issue 1
Abstract
Aim. Optimization of the nutrient medium (NM) for cultivation of Pseudomonas maltophilia ONU-329 strain – sorbents of heavy metals ions and destructors of oil hydrocarbons to obtain the maximum amount of biomass of bacteria and biofloccules. Methods. Optimization of the NM for the cultivation of bacteria P. maltophilia ONU-329 was performed using multifactorial experiment with subsequent mathematical processing of the data by the method of orthogonal Latin squares. Cultivation of bacteria was carried out in incubator shaker New Brunswick Scientific INNOVA 43R Incubator Shaker at 150 rpm for 48 hours at temperature +29.0 °С. Mathematical processing of experimental data was performed by calculating the effects of impact factors, after conducting the analysis of variance, adapted to the method of Latin squares. Results. To accumulate the maximum amount of biomass of P. maltophilia ONU-329 and a large number of biofloccules in the process of purification from heavy metals there were selected the conditions of cultivation of bacteria – composition NM: KH2PO4 – 1.5 g/l, Na2HPO4 – 3.0 g/l, NaCl – 5.0 g/l, NH4Cl – 1.0 g/l, peptone – 10.0 g/l, glucose 2.0 g/l, yeast extract – 5.0 g/l, distilled water or water, 1 litre; pH 7.2; temperature +29 °С. Conclusion. The experimental results on the study of indicators of optical density and number of colonies grown on medium MPA, using the methods of optimization based of the matrix of Latin squares, allowed to determine the best conditions for the accumulation of biomass of P. maltophilia ONU-329 strain.The obtained culture medium contributes to the increase of biomass of P. maltophilia ONU-329 strain and increases the number of biofloccules in 1.7 times.
Authors and Affiliations
N. Yu. Vasylieva, О. G. Gorshkova
Keywords
Related Articles
- EP ID EP318914
- DOI 10.18524/2307-4663.2016.1(33).65374
- Views 105
- Downloads 0
ANTIBIOTIC RESISTANCE AND BIOFILM FORMATION CHARACTERISTICS OF THE STRAINS KLEBSIELLA PNEUMONIAE ISOLATED IN CHILDREN WITH CONGENITAL HEART DISEASE
Klebsiella pneumoniae is one of the main pathogens that causes from 2 to 20% of all infectious complications. Nowadays, the strains of K. pneumoniae resistant to carbapenems have been registered. The mechanisms that caus...
EVALUATION OF EPIZOOTIC SITUATION WITH MAREK’S DISEASE VIRUS IN UKRAINE
Aim. To evaluate epizootic situation of Marek’s disease virus in Ukraine. Methods. Poultry with characteristic clinical features to Marek’s disease virus were investigated by histopathological analysis to prove the resea...
ISOLATION OF CU (II) FROM AQUEOUS SOLUTIONS BY IMMOBILIZED BACTERIAL CELLS OF THE GENUS PSEUDOMONAS
Aim. To develop a method for purifying water from Cu (II) using bacteria of the genus Pseudomonas immobilized on natural sorbents. Methods. For research use nonpathogenic bacterial strains P. cepacia ONU327, P. fluoresce...
ISOLATION OF AN α-GALACTOSIDASE FROM BIFIDOBACTERIUM LONGUM ЛМ-6 AND IDENTIFICATION OF FUNCTIONAL GROUPS OF THE CATALYTIC CENTER OF ENZYME
Aim. Receiving enzyme of an α-galactosidase of Bifidobacterium longum LM-6. Identification of the functional groups of the catalytic site of the enzyme. Methods. Cultivation and accumulation of biomass of bifidobacteria....
MUTAGENIC ACTIVITY OF TILORONE – ACTIVE PHARMACEUTICAL SUBSTANCE OF AMIXIN IN MICROTITER PLATE VARIANT OF AMES TEST
Aim – to discover potential mutagenic activity of tilorone – active pharmaceutical substance of amixin. Methods. Mutagenic activity of tilorone was studied in the Ames test on Salmonella typhimurium ТА 98 (frame-shift mu...