OPTIMIZATION OF THE NUTRIENT MEDIUM FOR CULTIVATION OF PSEUDOMONAS MALTOPHILIA ONU329 STRAIN – SORBENT OF HEAVY METALS IONS AND DESTRUCTOR OF OIL HYDROCARBONS
Journal Title: Мікробіологія і біотехнологія - Year 2016, Vol 0, Issue 1
Abstract
Aim. Optimization of the nutrient medium (NM) for cultivation of Pseudomonas maltophilia ONU-329 strain – sorbents of heavy metals ions and destructors of oil hydrocarbons to obtain the maximum amount of biomass of bacteria and biofloccules. Methods. Optimization of the NM for the cultivation of bacteria P. maltophilia ONU-329 was performed using multifactorial experiment with subsequent mathematical processing of the data by the method of orthogonal Latin squares. Cultivation of bacteria was carried out in incubator shaker New Brunswick Scientific INNOVA 43R Incubator Shaker at 150 rpm for 48 hours at temperature +29.0 °С. Mathematical processing of experimental data was performed by calculating the effects of impact factors, after conducting the analysis of variance, adapted to the method of Latin squares. Results. To accumulate the maximum amount of biomass of P. maltophilia ONU-329 and a large number of biofloccules in the process of purification from heavy metals there were selected the conditions of cultivation of bacteria – composition NM: KH2PO4 – 1.5 g/l, Na2HPO4 – 3.0 g/l, NaCl – 5.0 g/l, NH4Cl – 1.0 g/l, peptone – 10.0 g/l, glucose 2.0 g/l, yeast extract – 5.0 g/l, distilled water or water, 1 litre; pH 7.2; temperature +29 °С. Conclusion. The experimental results on the study of indicators of optical density and number of colonies grown on medium MPA, using the methods of optimization based of the matrix of Latin squares, allowed to determine the best conditions for the accumulation of biomass of P. maltophilia ONU-329 strain.The obtained culture medium contributes to the increase of biomass of P. maltophilia ONU-329 strain and increases the number of biofloccules in 1.7 times.
Authors and Affiliations
N. Yu. Vasylieva, О. G. Gorshkova
Keywords
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- EP ID EP318914
- DOI 10.18524/2307-4663.2016.1(33).65374
- Views 123
- Downloads 0
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