Optimized extraction and purification technology of Gekko sulfated glycopeptide based on its anticancer activities
Journal Title: Traditional Medicine Research - Year 2017, Vol 2, Issue 4
Abstract
Objective: To optimize the extraction and purification technologies of Gekko sulfated glycopeptide based on the content of glycopeptide, removal ratio of proteins, and anticancer activities. Methods: Different extraction methods, namely, water extraction, ultrasonic extraction, enzymatic hydrolysis-water extraction, and enzymatic hydrolysis-ultrasonic extraction were considered to determine the best extraction method. Single factor and orthogonal experiments were performed to determine the optimum extracting conditions. Sevage, enzymatic hydrolysis-Sevage, trichloroacetic acid (TCA), TCA-Sevage and enzymatic hydrolysis-TCA methods were tested to determine the best deproteinization method. The glycopeptide content and protein removal ratio were analyzed by the phenol-sulfuric acid and Coomassie Brilliant Blue methods. Results: Gekko sulfated glycopeptide obtained by water extraction could inhibit the proliferation of HepG2 and SKBR3, as well as promote that of lymphocytes. The glycopeptide content was 4.049% in the optimal extracting condition of a triple decoction extraction for 1 hour each time with a material to solvent ratio of 1:15. The enzymatic hydrolysis-TCA method was found to be the optimal deproteinization method, with a protein removal ratio of 50.46%, glycopeptide content of 14.27%, and inhibitory ratio on human HepG2 cells of 49.06%. Conclusion: This extraction and purification technique for Gekko sulfated glycopeptide is reasonable, feasible, and provides a scientific basis for industrial production.
Authors and Affiliations
Nan Nan, Liu Chang, Xu Jing, Meng Li-Na, Liu Xiao-Hui, Wu Xiong-Zhi, Chen Dan, Zhou Jing
Keywords
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- EP ID EP45253
- DOI 10.12032/TMR201708057
- Views 262
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