Optimized Protocol for PFGE Analysis of Anginosus (milleri) Streptococci

Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 1

Abstract

[i]Streptococcus anginosus (milleri ) [/i]is a diverse group of gram positive bacteria. Molecular methods to establish relationship between strains are poorly developed. Therefore, main tool to study genetic variability is restriction fragment length polymorphism combined with pulsed field gel electrophoresis (RFLP-PFGE). In this communication, we present optimized protocol for [i]S. anginosus[/i] PFGE analysis.

Authors and Affiliations

Katarzyna Obszańska, Izabela Kern-Zdanowicz

Keywords

Related Articles

<strong><em>Streptococcus anginosus</em> (<em>milleri</em>) group strains isolated in Poland 1996-2012 and their antibiotic resistance patterns</strong>

[i]Streptococcus anginosus[/i], [i]Streptococcus intermedius[/i] and [i]Streptococcus constellatus[/i] form group of related streptococcal species namely Streptococcus Anginosus Group (SAG). The group, previously called...

Safety Evaluation of Enterocin Producer Enterococcus sp. Strains Isolated from Traditional Turkish Cheeses

The purpose of this study was to determine the antimicrobial activity and the occurrence of bacteriocin structural genes in Enterococcus spp. isolated from different cheeses and also investigate of their some virulence f...

Outpatient Antibiotic Consumption Fluctuations in a View of Unreasonable Antibacterial Therapy

Unreasonable antibacterial therapy is suspected to be the main reason of emergence of multi-resistant bacteria. The connection between seasonal variability of antibiotic use and reasonable antibacterial therapy has been...

Prevalence of Urogenital Mycoplasmas Among Men with NGU in Upper Silesia, Poland. Preliminary Study.

The prevalence of urogenital mycoplasmas in men with NGU in Upper Silesia (Poland) was studied. Mycoplasmas were detected in 36.7% men ([i]Ureaplasma parvum[/i] and [i]Mycoplasma genitalium[/i] were found in 30% and 1...

The Diversity, Growth Promoting Abilities and Anti-microbial Activities of Bacteria Isolated from the Fruiting Body of Agaricus bisporus

Agaricus bisporus plays an important role in ecological processes and is one of the most widely cultivated mushrooms worldwide. Mushroom growth-promoting bacteria have been isolated from casing soil and compost, but micr...

Download PDF file
  • EP ID EP59283
  • DOI -
  • Views 99
  • Downloads 0

How To Cite

Katarzyna Obszańska, Izabela Kern-Zdanowicz (2015). Optimized Protocol for PFGE Analysis of Anginosus (milleri) Streptococci. Polish Journal of Microbiology, 64(1), 61-64. https://europub.co.uk./articles/-A-59283