Optimizing Phenol-Chloroform Extraction Method for Human Papilloma Virus (HPV) Genomic DNA Isolation from Fresh Cervical Tissue
Journal Title: Indian Journal of Anatomy - Year 2017, Vol 6, Issue 2
Abstract
Our aim was to optimize a cost effective, efficient protocol for genomic DNA extraction of HPV from exfoliated cells of cervix. The final goal was to get a high quality and quantity DNA yield. The DNA obtained was confirmed by horizontal gel assembly and visualizing the same by Gel Doc System. The optimized protocol for genomic DNA extraction of HPV is good for laboratory with low funds or budget.
Authors and Affiliations
B. R. Sontakke
Keywords
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- EP ID EP467632
- DOI 10.21088/ija.2320.0022.6217.12
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