OPTIMUM CONDITIONS FOR N-ACETYL GLUCOSAMINE PRODUCTION FROM TIGER SHRIMP (PENAEUS MONODON) SHELL BY SERRATIA MARCESCENS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objective: The aim of this research was to determine the optimum condition for Serratia marcescens to produce optimum amount of N-acetyl glucosamine using chitin isolated from tiger shrimp (Penaeus monodon) shells.Methods: This research was conducted using submerged fermentation method. The treatments used were various fermentation temperatures (20, 30, and 37°C), pH (6, 7, and 8), and incubation period (2, 4, 6, and 8 days).Results: Chitinolytic index of Serratia marcescens was 2.203±0.59 after 2 days of incubation. Optimum temperature for N-acetyl glucosamine production using S. marcescens was at 30°C. Optimum pH and incubation period for N-acetyl glucosamine production were at pH 8 and 6 days of incubation period.Conclusion: S. marcescens is able to ferment chitin from shrimp shell to produce N-acetyl glucosamine of 41,166.11±4,480.59 mg/l at optimum fermentation condition.
Authors and Affiliations
Yuniwaty Halim, Hardoko Hardoko, Ratna Handayani, Vania Lucida
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