Oral Candidiasis Susceptibility of Mice Lacking Interferon Regulatory Factor 3, A Preliminary Report
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 14, Issue 4
Abstract
The purpose of this research was to evaluate the significance of interferon regulatory factors 3 to the susceptibility of oral candidiasis in mice. Materials and Methods: IRF-3KO and C57BL/6 mice were injected with cortisone acetate subcutaneously in the dorsum of the neck and were used as the experimental groups. Mice with no cortisone acetate treatment were used as controls. Each mouse was inoculated by swabbing the oral cavity with a cotton swab soaked in PBS containing 108 CFU/ml of C. albicans. On days 1, 3, and six, a swab from the oral cavity of all mice was performed and plated on YPD agar to determine fungal burden. On day six post-infection, mice were sacrificed, and tongues were extracted and submitted for histology. Results: The control mice, without cortisone acetate treatment had maintained their weight throughout the experiment. When both groups were treated with cortisone acetate, considerable body weight was lost. At day 3, 5, and 6, the IRF-3KO mice which were treated with cortisone acetate, a significant weight loss was observed compared to C57BL/6 mice. Mice that were treated with cortisone acetate had significant fungal burden at day 3 and 6. Microscopic analysis showed that fungal infection and hyphal formation invaded the superficial epithelial layer of the mucosa and the papillae in both experimental groups. The infiltration of neutrophils and eosinophils was observed more in IRF-3KO mice which were treated with cortisone acetate compared C57BL/6 mice that received the same treatment. In conclusion, IRF-3 deficient mice are more susceptible to oral candidiasis if treated with cortisone acetate.
Authors and Affiliations
Fahd Alsalleeh
Keywords
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- EP ID EP585928
- DOI 10.26717/BJSTR.2019.14.002571
- Views 141
- Downloads 0
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