Orgfanization and variability of the 5S rDNA intergenic spacer of Lathyrus venetus
Journal Title: Вісник Українського товариства генетиків і селекціонерів - Year 2015, Vol 13, Issue 1
Abstract
Aim. In populations of Lathyrus venetus (Mill.) Wohlf., which is an endangered species included in the Red Book of Ukraine, the majority of plants are hybrids between the original species and the related L. vernus. Therefore, the use of molecular markers, such as intergenic spacer region (IGS) of 5S rDNA, appears to be necessary for the assessment of genetic homogeneity of L. venetus populations and for development of a rational strategy of this species’ preservation. Methods. PCR amplification, cloning and sequencing of the 5S rDNA IGS of L. venetus. Results. It was found that two variants of 5S rDNA repeats are present in the genome of L. venetus. The IGS size is 158 and 162 bp, and the content of GC pairs amounts to 22,8–22,9 %. Typical for angiosperms RNA polymerase III promoter elements were detected in the IGS fragment preceding the 5S rRNA coding sequence. Among representatives of 17 genera of Fabaceae the highest level of IGS sequence similarity of 85,4–86,7 % was revealed between L. venetus and Pisum sativum, which belong to tribe Fabeae. Conclusions. High variability of the 5S rDNA IGS makes them a convenient molecular marker for elucidation of phylogenetic relationships between genera and species of tribe Fabeae. Keywords: 5S rDNA, molecular markers, endangered species, Lathyrus.
Authors and Affiliations
Y. O. Tynkevich, A. O. Nevelska, I. I. Chorney, R. A. Volkov
Keywords
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