Osteoblast And Osteoclast Ratio Analysis Based On Bacterial Turbidity Grades On Periapical Bone Resorption induced byFusobacterium nucleatum
Journal Title: Scholars Journal of Dental Sciences - Year 2015, Vol 2, Issue 1
Abstract
Abstract: Bacterial infection that accumulated in the pulp of the tooth generally provokes periapical lesions and bone resorption. These periapical lesions as results of necrotic pulp mostly inducted by anaerobic bacterial infection. One of the most prevalent anaerobic bacteria in the root canal systems is Fusobacterium nucleatum (Fn). Bone resorption is influenced by osteoclast activating factor such as prostaglandin, bacterial endotoxins, as well as activator complement products that consist of cytokines including as IL-1β, IL-1α, TNF-α, TNF-β, and IL-6. The main purpose of this study is to determine the difference between ratio numbers of osteoblast and osteoclast in the periapical tissue that induced by Fn with various grades of turbidity. This study design used an experimental laboratory. A total 16 male Wistar rats were divided into 3 groups. Group A: the teeth were induced by Fn with 10x108 turbidity; Group B: the teeth were induced by Fn with 10x1010 turbidity; and Group C: a control group without any bacterial induction. After euthanasia procedure was applied to the all samples 3 weeks after induction, a histological examination of their periradicular tissues was conducted. The analysis reveals that number of osteoclast cells is significantly higher than osteoblast cells in the treated groups with 10x108 and 10x1010 turbidities. Meanwhile, the study also shows that number of osteoclast cells is significantly higher in group with 10x108 turbidity than ingroup with 10x1010 turbidity. An increased number of osteoclast cells is also detected in the area with periapical bone resorption after being inducted by Fn.
Authors and Affiliations
Juni Jekti Nugroho, Andi Sumidarti, Latief Mooduto, Suryani As’ad
Keywords
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