OVARIECTOMISED AND LONG TERM INDUCED (OLI) RAT MODEL FOR PRECLINICAL ASSESSMENT OF OSTEOPOROTIC THERAPEUTICS.
Journal Title: Journal of Drug Delivery and Therapeutics - Year 2017, Vol 7, Issue 2
Abstract
Osteoporosis affects millions of people, especially the aged population and numerous researchers focus on developing novel pharmacotherapeutic agents for the prevention and treatment of osteoporosis. Animal models have become an indispensable part of the pre-clinical assessment of osteoporotic therapeutics and rat models have been widely preferred. The majority of studies so far reported have used young or short-term induced rat models, but they do not mimic the pathological condition in senile osteoporosis. Hence this study has focused on developing an ovariectomised and long term induced (OLI) aged rat model for the preclinical assessment studies. Long term osteoporosis was induced in Wistar rats by ovariectomy followed by the maintenance period of 10 months. Histological evaluation and Micro-CT analysis substantiated the extensive bone loss in the OLI rats compared to Control and ovariectomised and short term induced (OSI) rat model. OLI rats also exhibited impairment in the healing of 3 x 1.5 mm femoral cortical bone defect even after 8 weeks of surgery. Histomorphometric analyses indicated that OLI rats exhibited a poor regeneration efficiency (RE) ratio of 0.67 ± 0.2 compared to 0.95 ± 0.1 in the Control group. Chronic bone loss in the OLI rats together with the impaired osteogenic efficacy has been successfully demonstrated which proposes OLI rat models to be utilized for investigating novel pharmacotherapy’s or novel biomaterials intended for osteoporotic applications.
Authors and Affiliations
Chandran Sunitha
Keywords
Related Articles
- EP ID EP294661
- DOI 10.22270/jddt.v7i2.1400
- Views 97
- Downloads 0
NEW SLANT ON HYPERLIPIDEMIA: PREVENTION BY VITAMIN B-3 AND COMMON FRUIT ZIZIPHUS JUJUBA
Coronary artery disease (CAD) is complication of primary or secondary hyperlipidemia. Etiological factors for hyperlipidemia include inherited genetic defects in low density lipoproteins (LDLs), persons accustomed to sit...
FORMULATION AND EVALUATION OF AN INJECTABLE SOLUTION AS A DOSAGE FORM
The present study will outline formulation and the evaluation methods of injectable dosage form. The various initial formulations of the developed and those are examined for drug release profile, bioavailability, and cli...
EXPLORATION OF ANTI-HYPERGLYCEMIC AND HYPOLIPIDEMIC ACTIVITIES OF ETHANOLIC EXTRACT OF TINOSPORA CARDIFOLIA (WILD)HOOK WHOLE PLANT IN ALLOXAN INDUCED DIABETIC RATS
Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for diabetes mellitus. The aim of present study was to demonstrate the hypoglycemic and anti-diabetic activity of the E...
CHITOSAN: A REVIEW ON ITS VARIED NOVEL THERAPEUTIC AND INDUSTRIAL APPLICATIONS
Chitosan and its nanoparticles, which are being studied extensively to understand their varied and unique functional properties, have attracted considerable interest due to their biological activities and potential appli...
A REVIEW ON THERAPEUTIC APPLICATIONS OF PHYTOSOMES
Ethnobotanical plants have been used in the treatment of various diseases since antiquity. The phytoconstituents have pharmacological activities such as anti-tumor, anti-inflammatory, antinociceptive, anti-obesity, therm...