Over expression of Nrf2 in Umbilical Cord-derived Mesenchymal Stem Cells Up regulates Cytoprotective Genes, TXNRD1 and GCLC
Journal Title: Iranian Journal of Blood and Cancer - Year 2015, Vol 7, Issue 3
Abstract
Background: Mesenchymal stem cells (MSCs) are ideal cells for cell and gene therapy. However, the low survival of MSCs after transplantation has limited their applications. We aimed to evaluate the expression of cytoprotective genes including NQO1, TXNRD1, HO-1, GCLC following the over expression of Nrf2 in umbilical cord-derived MSCs (UC-MSCs). Methods: 3-5 passages of UC-MSCs were cultured. Recombinant vector containing Nrf2 (pcDNA3.1-Nrf2) and empty vector were transfected into MSCs using FuGENE HD. After exposing the cells to UV light stress, RNA extraction and cDNA generation were performed. Using Primer3, software specific primers were designed for Nrf2, NQO1, TXNRD1, HO-1, and GCLC genes and the expression of these genes was evaluated by RT-PCR. The results were semi-quantified and analyzed statistically utilizing Image J2x software and ANOVA test. Results: The expression of Nrf2 was up-regulated in UC-MSCs after transfection with pcDNA3.1-Nrf2 (P<0.01). Over expression of TXNRD1 and GCLC were also confirmed in these transfected cells (P<0.05 and P<0.01, respectively). However, expression of NQO1 and HO-1 did not alter in the transfected cells with pcDNA3.1-Nrf2 compared with those transfected with empty vector (P>0.05). Conclusion: Over expression of Nrf2 resulted in the over expression of TXNRD1 and GCLC in MSCs and might be explained by the fact that a known part of Nrf2 cytoprotective mechanisms is controlled by the expression of these genes. Keywords: Mesenchymal Stem Cells, Over-expression, Nrf2, TXNRD1, GCLCKeywords: Educational games, Computer games, Self-Efficacy, Hemophilia.
Authors and Affiliations
H Mehrabi Habibabadi, F. Amiri, E Moslemi, M Habibi Roudkenar
Keywords
Related Articles
- EP ID EP361293
- DOI -
- Views 95
- Downloads 0
Comparative Study of Four Platelet-Rich Plasma Methods for Preparing Platelet Concentrates
Background: Platelet preparations have been used for a wide variety of clinical applications such as hemorrhage, tissue engineering and cellular therapy. Platelet concentrates can be prepared by the apheresis method or f...
Obituary: Professor Parvaneh Vossough
This article has no abstract.
Relation between Estrogen and Progesterone Receptor Status with p53, Ki67 and Her-2 Markers in Patients with Breast Cancer
Background: Breast cancer is the most common cancer in women, containing approximately one third of all illnesses in women. Assessment of molecular markers is valuable in predicting the outcome of disease and decision ma...
A Rare Presentation of Arterial Thrombosis after Venous Thrombosis in a Patient with Gastric Cancer
Background: Venous thromboembolism, which comprises deep vein thrombosis and pulmonary embolism and is a significant cause of morbidity and mortality among patients with cancer. The aim of the present study was to report...
Comparative Effect of Chamomile Mouthwash and Topical Mouth Rinse in Prevention of Chemotherapy-Induced Oral Mucositis in Iranian Pediatric Patients with Acute Lymphoblastic Leukemia
Background: Oral mucositis afflicts more than 3/4 of patients with cancer under chemotherapy. In acute cases it could lead to brain damage caused by hypoxia and even death due to airway obstruction and reduction of chemo...