Papaya extract as new endodontic irrigant
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 9
Abstract
Introduction: The purpose of the study was to compare the antimicrobial efficacy of crude papaya extract with 5.25% Sodium hypochlorite(NaOCl) and 2% Chlorhexidine(CHX) against Enterococcus faecalis and Candida albicans at 1 min, 5 min and 15 min time intervals. Methods: #72 absorbent paper points were immersed in experimental suspensions (E. faecalis and C. albicans) for 5 mins and placed on test tubes to cover with irrigants (NaOCl, CHX, papaya extract, and distilled water. Distilled water was used as negative control. At 1 min, 5 mins and 15 mins intervals, 18 absorbent points for each irrigants were removed from contact with irrigants, individually transported to immerse in Letheen broth and incubated at 37ºC for 48 hr. Microbial growth was analyzed by turbidity of culture medium and measured by spectrophotometer at 560 nm. Inoculum obtained from Letheen broth was serially diluted for spread plate. Colony Count was done for viable cell count. Result: The result of turbidimetric measurement showed crude papaya extract to be effective against E. faecalis after 5 mins whereas it was effective against Candida albicans at all time intervals. The result of viable cell count of crude papaya extract shows gradual decrease of colony count from 1 min to 15 min against E. faecalis and Candida albicans. Conclusion: Papaya extract demonstrated potent antimicrobial activity against E. faecalis and Candida albicans comparable to 5.25% NaOCl and 2% CHX at 5 min and 15 min time intervals.
Authors and Affiliations
Dr Snigdha Shubham, Dr Praveen Singh Samant, Rita Khanal, Vanita Gautam, Ourvind Jeet Singh Birring, Chetna Arora, Ravish Mishra, Laxmi Kandel
Keywords
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- EP ID EP395386
- DOI 10.9790/0853-1709062328.
- Views 63
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