Partial Characterization of Acid Phosphatase from Ginger (Zingiber Officinale) Rhizomes
Journal Title: GJRA-Global Journal For Research Analysis - Year 2015, Vol 4, Issue 5
Abstract
Acid phosphatase has been isolated and purified from ginger rhizome following conventional methods of protein purification with a molecular weight of 46kDa as judged by SDS-PAGE. The enzyme was found to be more stable around pH 5-9 and it was less stable at acidic pH (3-4) and the alkaline pH (10-11). The enzyme was completely inactive when exposed to 900C for 30min. The Km value of acid phosphatase was found to be 0.083M calculated from Line weaver- Burk plot and showed broad substrate specificity. Of the metals tested, only Zn2+ at 10mM concentration inhibited the enzyme to an extent of 60% and the rest of the metals tested were ineffective either in activating or inhibiting the enzyme. The activity of the enzyme was unaffected when it was exposed to 0.1% urea and guanidine hydrochloride. It was, however, inactivated to an extent of 50% when exposed to SDS (0.1%).
Authors and Affiliations
Amrutha Mani Prabha Jaladi, Muni Kumar Dokka, Siva Prasad Davuluri
Keywords
Related Articles
- EP ID EP584204
- DOI -
- Views 94
- Downloads 0
ANATOMIC VARIATIONS IN THE MIDDLE CEREBRAL ARTERY IN HUMAN CADAVERS
INTRODUCTION: Middle cerebral artery (MCAis the largest branch among all the branches of internal carotid artery,abnormal development of which in the embryonic period can lead to developmental anomalies of the cerebral a...
A COMPARISON OF ASPIRATION WITH NON ASPIRATION TECHNIQUE IN FINE NEEDLE CYTOLOGY OF THYROID LESIONS
Back ground: Fine needle cytology is a valuable adjunct to preoperative diagnosis of thyroid lesions and it can helps in assessment of benign and malignant condition. Aims and objectives: To compare the efficacy of fine...
THE ROLE OF HEPARIN IN TREATMENT OF EXPERIMENTAL PERITONITIS
Background: To produce peritonitis experimentally in albino rats by creation of a necrotic loop of terminal Ileum. Study of peritonitis and gross changes in peritoniteal cavity and to study the effect of single dose hepa...
Awareness of Women on Child Care Practices in Gulf of Mannar, Tamil Nadu
Health seeking behaviour of parents for the child is influenced by a large number of factors apart from knowledge and awareness. Objective: To study the awareness level of women on child care practices in Gulf of Mannar,...
A Cross Sectional Study of Teaching and Evaluation Methodology in Physiology
Background: Continuous monitoring and improvement is necessary for education technology. The student’s feedback is inevitable, inexpensive and valuable tool to improve teaching and evaluation methods. Their feedback coul...