Pаthоlоgenic changes in the brain of mice while modeling of ischemia with an associated viral infection
Journal Title: Український неврологічний журнал - Year 2019, Vol 0, Issue 2
Abstract
Objective — to investigate structural changes in the brain cortex of mice exposed to fat diet, herpes infection and ischemia. Methods and subjects. We had 194 mice (Balb/c) for the experiment, distributed into 7 groups (6 + 1 control group): 1st group (n = 52) with fat diet; 2nd group (n = 30, 7 died) with HSV1; 3d group (n = 30) with ОGСА; 4th group (n = 20, 10 died) with HSV1 and ОGСА; 5th group (n = 5) with HSV1 and fat diet with cholesterol; 6th group (n = 16, 10 died) with HSV1, fat diet, and ОGСА.The neuronal neocortex density of the parietal, temporal cortex and hippocampus were morphometrically evaluated on microscopic specimens. Results. In the course of experiment, we discovered the main zones and anatomical formations of brain which received the most damage. Changes were mainly found in the neocortex of the parietal, temporal lobes and hippocampus. To assess the type of neuronal brain damage, the main changes were divided into focal and diffuse. Later in the study, we attempted to investigate the dynamics of changes in the density of neurons with degenerative changes in the parietal and/or temporal cortex of the brain, in the group with fat diet, HSV1 and ОGСА. Conclusions. Based on microscopic examination of the temporal and/or parietal cortex in laboratory animals, we established that the combination of factors such as fat diet, neuroinfectious diseases and ischemia resulted in increase in the density of neurons with degenerative changes and on the dynamics of their development. Using morphometric study of the cerebral cortex in laboratory mice, we determined a statistically significant increase in ischemic neuronal damage resulted in condition of an experimental model with infection with HSV‑1 and fat diet, and under the condition of ОGСА of diffuse character. The increase of the degree of neocortex damage in mice with herpes infection and fat diet evidences the interrelation of them.
Authors and Affiliations
N. S. Тurchynа, S. I. Sаvоskо, Т. М. Chеrеnkо, S. L. Ribаlkо, D. B. Stаrоsilа
Keywords
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- EP ID EP670645
- DOI 10.30978/UNJ2019-2-57
- Views 96
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