PCR Methods for Detecting Bovine Respiratory Pathogens
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2018, Vol 11, Issue 4
Abstract
Bovine Respiratory Disease (BRD) is an important disease in cattle production, BRD may be associated with one or more pathogens, of which Mycobacterium bovis, Mycoplasma bovis, and Klebsiella pneumoniae are three important pathogens. Fast and accurate detection methods are important for preventing and controlling BRD. This review focuses on the PCR detection methods for the above three pathogens in recent years.Bovine Respiratory Disease (BRD) is an important disease in cattle production, causing serious economic losses world widely [1]. The occurrence of BRD is a combination of multiple factors and may be associated with one or more pathogens [2]. Among them, Mycobacterium bovis, Mycoplasma bovis, and Klebsiella pneumoniae are three important pathogens. Mycobacterium bovis can infect many kinds of animals. Besides cattle, there are 50 kinds of vertebrates such as humans. Sick animals showed a gradual loss of body weight, anemia and cough. Cattle with active tuberculosis are the main source of infection. Their respiratory tract carrys bacteria, which are excreted from coughing and sneezing [3]. Mycoplasma bovis is one of the main pathogens involved in cattle pneumonia. It was found that 5.5% of the nasal swabs from cattle with respiratory symptoms were positive for Mycoplasma bovis [4]. Klebsiella pneumoniae, an important conditional pathogen, mainly exists in the intestine, respiratory tract and urogenital tract [5]. The incidence of respiratory and urinary tract is the highest. Aslan et al. isolated bacteria from bovine upper respiratory tract infections and found that Klebsiella pneumoniae accounted for 20% [6]. PCR technology is a molecular biotechnology in which DNA of pathogenic microorganisms is expanded to conventional detectable levels in vitro. Quan Z et al. designed a multiplex PCR with primers targeting the 16S rRNA, Rv3873 and a 12.7-kb fragment in the genomes of a Mycobacterium tuberculosis complex to differentiate Mycobacterium bovis from Mycobacterium tuberculosis and NTM species [7]. Gioia et al. developed and validated a multitarget PCR assay that can discriminate between Acholeplasma and Mycoplasma and identify Mycoplasma bovis [8]. Turton et al. identified and typed Klebsiella pneumoniae by PCR using capsular type-specific, variable number tandem repeat and virulence gene targets [9]. Fonseca et al. established a one-step multiplex PCR to identify klebsiella pneumoniae, klebsiella variicola and klebsiella quasipneumoniae in the clinical routine [10].
Authors and Affiliations
Yuhui Dong, Lijia Luo, Xiangmei Zhou
Keywords
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- EP ID EP586176
- DOI 10.26717/BJSTR.2018.11.002145
- Views 122
- Downloads 0
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