Pengembangan Mikroskop dengan Mikrokontroler dan Cahaya Monokromatis untuk Mendeteksi Parasit Malaria
Journal Title: Jurnal Teknologi Laboratorium - Year 2017, Vol 6, Issue 2
Abstract
Malaria still become one of major health burden in Indonesia especially in remote areas of east Indonesia. Golden standard of malaria parasite detection is still microscopic technique using policromatich lightsource whether from halogen or natural light source. Microscopic technique have alot of benefit but still have weakneses, such as time consumming and bias on the reading by microscopist, because of artifact in the image. Aims of this study was to designed malaria parasites detection tool that is robust, fast, convenient and clear by minimizing artifact on slide. Design of this study was laboratory experimental which modified simple microscope into automatic microscope with table movement and webcam recording using microcontroller and monochromatic light source.Wavelength of the light sources were 402nm(blue), 532 nm (green) and 650 nm (red), intensity of each sources were differ. The reading of the slide image was conducted by two certified microscopist, whom read 60 images of thick and thin slide with three different live stage of Plasmodium falciparum live, which werering, trophozoit and schizont. This study showed that modification of microscope was succeeded with automatic movement and webcam recording, process time in one step movement and recording approximately 10 seconds or 17minutes for 100 field of view as confirmation process.Monocromatic light source has proven to give a clear and contrast field of view when the intensities were higher than 40 mW and the certified microscopist able to identified Plasmodium falciparum parasites. Data analysis of microscopist reading used non parametric statistic friedman by SPSS showed that correlation between images using monocromatics and policromatic lights have meaningless differences in a thick and thin slide. However, hemozoin as marker of plasmodium falciparum parasite was less detected by monochromatic light used in this study.
Authors and Affiliations
Ida Susanti, Sarwo Handayani, Riyanti Ekowatiningsih, Budi Prasetyorini, Endah A Yusnita, Donni Agus Ardianto, Sastra K Widjaya
Keywords
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Pengembangan Mikroskop dengan Mikrokontroler dan Cahaya Monokromatis untuk Mendeteksi Parasit Malaria
Malaria still become one of major health burden in Indonesia especially in remote areas of east Indonesia. Golden standard of malaria parasite detection is still microscopic technique using policromatich lightsource whet...