Phenolic Contents and In vitro Pharmacological Activities of Methanolic Extract of Pterocarpus erinaceus Poir. Stem Bark (Fabaceae)
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 10, Issue 2
Abstract
Aim: To investigate the cytotoxic effect of methanol extract of Ptreocarpus erinaceus stem barks against cancer lines (Leukemia cell K562 and lung small cells A549) and its inhibition effects against NF-кB activation and lipoxygenase enzyme. Moreover, phenolic compounds, tannins and flavonoids amounts have been determined. Study Design: In vitro evaluation of cytotoxic activity, inhibitor effect against NF-кB activation and lipoxygenase; and quantitative determination of total phenolic contents. Place and Duration of Study: Laboratory of Département de médecine-pharmacopée traditionnelle and Laboratoire of Biologie Moléculaire et Cellulaire du Cancer (February – November 2013). Methodology: The cytotoxicity of methanol extract from stem bark of this plant against cancer cell lines (Leukemia cell K562 and lung small cells A549), and its inhibition of NF-кB activation in K562 cells and lipoxygenase were studied. Additionally phytochemical composition including phenolic, tannins, flavonoids and flavonols was determined by using standard methods. Results: At 50 µg/mL, the methanol extract of P. erinaceus inhibited the growth of K562 and A549 cells with inhibition percentage of 46.2±4.0 and 59.4±4.5% respectively. Extract inhibited NF-кB activation at 50 µg/mL (86.5±2.7%), and lipoxygenase activity was significantly inhibited by extract at 100 µg/mL (97.7±0.5%). Phytochemical analysis shows the presence of phenolic compounds, tannins, flavonoids and flavonols in a methanolic extract of Pterocarpus erinaceus stem bark. Conclusion: Pterocarpus erinaceus stem bark showed significant cytotoxic effect and inhibited NF-кB activation and lipoxygenase. These pharmacological effects could be due to the presence of phenolic compounds, tannins, flavonoids and flavonols.
Authors and Affiliations
Ouédraogo Noufou, W. R. Sawadogo, A. Tibiri, M. Lompo, M. G. Dijoux, I. P. Guissou
Keywords
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- EP ID EP342385
- DOI 10.9734/BJPR/2016/23149
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