Phylogenetic analysis of HIV-1 variants of a comprehensive HIV clinic in Guatemala, through the subtyping of the pol-HIV-1 region sequences obtained during the period 2010-2015
Journal Title: Revista Científica Facultad de Ciencias Químicas y Farmacia - Year 2019, Vol 29, Issue 1
Abstract
The HIV genome contains nine genes, three of these genes (gag, pol, and env) encode structural proteins. There are two main variants of this virus, HIV-1 and HIV-2. The first one (HIV-1) is the cause of most infections worldwide, of which nine subtypes and 58 circulating recombinant forms (CRF) have been identified. In Central America, subtype B of HIV-1 is the cause of the majority of HIV positive cases. In Guatemala, it has been reported the presence of subtype B, recombinant forms BF1 and subtype C. However, no phylogenetic analysis has been performed to indicate the variants of this subtype. The aim of the study was to subtype 400 sequences of the pol region of HIV-1, of samples that were obtained from a care clinic during the period 2010 to 2015. To determine the different subtypes of HIV-1 present in Guatemala, the subtyping of the sequences obtained by the genotype test, in FASTA format, was performed with REGA HIV-1 Subtyping Tool - Version 3.0. In order to determine the relationship between HIV-1 variants, an alignment of sequences and phylogenetic trees was performed using the Neighbor Union and Maximum Likelihood method with 100 bootstrap replicas, with the MEGA 7.0.21 program. It was determined that the subtypes with the highest prevalence of the studied sequences are the subtype B (71.5 %), recombinant BD (16.75 %), and subtype B-like (7.75 %)
Authors and Affiliations
Oliva-Castillo, Ana; Lau-Bonilla, Dalia
Keywords
Related Articles
- EP ID EP675522
- DOI -
- Views 175
- Downloads 0
Equivalencia terapéutica in vitro de tabletas de enalapril maleato 20 mg de producción nacional
Los ensayos de equivalencia terapéutica tienen como objetivo demostrar que los medicamentos genéricos aportan la misma cantidad de principio activo en comparación con el medicamento innovador. El objetivo de la investiga...
Editorial 29 /2)
La actual coyuntura de emergencia sanitaria, ha verificado el valor del impacto de la inversión de tiempo y trabajo en la elaboración y actualización del sitio Web de la Revista Científica, situación que permitió estar...
Metabolic Profile of Medical Students Before and After Work Shifts at Roosevelt, San Juan de Dios, Regional de Cuilapa, Escuintla and Nacional de Antigua Hospitals
The long work shifts of 16 to 24 hours and up to 32 to 36 hours that doctors have in the hospitals, exert a negative effect on their physical and emotional health. Alterations have been described in the levels of glycae...
Caracterización de carbapenemasas en enterobacterias de muestras de pacientes que acudieron al Hospital General San Juan de Dios de la ciudad de Guatemala durante 2014 y 2015 Characterization of carbapenemases in Enterobacteriaceae of samples of patients who attended the San Juan de Dios General Hospital in Guatemala City during 2014 and 201
En salud pública a nivel mundial, la producción de carbapenemasas es actualmente el mayor problema de resistencia antimicrobiana. El objetivo de este estudio fue caracterizar las carbapenemasas en enterobacterias en paci...
Amylase production of strains of anamorphic fungi isolated from leaf litter of Quercus sp.
Anamorphic fungi are a group of microorganisms of great importance because they produce a wide variety of substances as part of their secondary metabolism, as well as various enzymes useful in the degradation of differen...