PHYTOCHEMICAL SCREENING, ANTIMICROBIAL, AND ANTIOXIDANT ACTIVITIES OF ROOT AND LEAF EXTRACTS OF LEUCAS ASPERA
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 3
Abstract
Objective: The present study was designed to evaluate the phytochemical screening, antimicrobial, and antioxidant activities from the roots and leaves of Leucas aspera. Methods: The phytochemical screening and Fourier transform infrared (FTIR) analysis of root and leaf extracts were studied using standard methods. The disk diffusion method was performed to analyze the antimicrobial activity of aqueous extract, methanol extract and hexane extract of root and leaf against to selected bacterial and fungal strains. Antibiotics, streptomycin and nystatin were used as standards for bacteria and fungi, respectively. The antioxidant activity of the extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and scavenging of H2O2 free radicals. Results: Preliminary phytochemical screening of extracts exhibited the presence of carbohydrates, proteins, amino acids, steroids flavonoids, terpenoids, saponins, alkaloids, tannins, and phlobatannins. Leaf extracts exhibited effective antibacterial and antifungal activities compared with root extracts against all the tested bacteria and fungi. ME of the leaves exhibited highest antibacterial activity against Staphylococcus aureus (12.8±0.31 mm), followed by Bacillus subtilis (11.4±0.3 mm), Escherichia coli (9.8±0.21 mm), and Pseudomonas aeruginosa (7.3±0.29 mm). Leaf extracts of L. aspera showed effective scavenging activity compared with root extracts. ME of the leaves showed maximum scavenging activities of 38.39 and 36.85%, respectively, against DPPH and H2O2 free radicals with half maximal inhibitory concentration values of 136.17 and 142.42 μg/ml. Conclusion: Phytochemical analysis and FTIR spectrum revealed that different plant secondary metabolites particularly alkaloids, terpenoids, and flavonoids could be responsible for antimicrobial and antioxidant activities of L. aspera leaf extracts.
Authors and Affiliations
VASUDHA K, ARCHANA D, MUTYALAMMA B, KISHORI B
Keywords
Related Articles
- EP ID EP603840
- DOI -
- Views 161
- Downloads 0
THE METAL COMPLEXES OF NOVEL SCHIFF BASE CONTAINING ISATIN: CHARACTERIZATION, ANTIMICROBIAL, ANTIOXIDANT AND CATALYTIC ACTIVITY STUDY
Objectives: The aim of our work was to synthesize novel mixed ligand-metal complexes and evaluation of antimicrobial, antioxidant assay, and analysis of catalytic oxidation of cyclohexane. Methods: The complexes were ch...
ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF LUDWIGIA PARVIFLORA L. AGAINST STANDARD BACTERIAL STRAINS AND COMPARISON OF ITS ACTIVITY WITH THAT OF STANDARD ANTIBIOTICS
Objective: The objective of this study was to evaluate the antimicrobial activity of methanolic extract of Ludwigia parviflora L. using standard bacterial strains and compare its activity with that of standard antibiotic...
THE EFFECT OF PUGUNTANO (CURANGA FEL-TERRAE [LOUR.]) EXTRACT ON ADIPONECTIN RECEPTOR (ADIPOR) IN RATS WITH TYPE 2 DIABETES MELLITUS
Objective: This study aimed to determine whether the antidiabetic effects of puguntano (Curanga fel-terrae [Lour.]) extract involve anti-inflammatory effects mediated through adiponectin receptors (AdipoRs). Methods: Ty...
BALANITES MAUGHAMII SPRAGUE (ZYGOPHYLLACEAE) IN TROPICAL AFRICA: A SYNTHESIS AND REVIEW OF ITS CHEMISTRY, PHARMACOLOGY, AND MEDICINAL POTENTIAL
Balanites maughamii is an important medicinal plant species in Southern Africa. This study is aimed at providing a critical review of the biological activities, phytochemistry, and medicinal uses of B. maughamii. Documen...
IS DOSE TITRATION REQUIRED FOR ANTIHYPERTENSIVE AGENTS IN GERIATRIC DIABETIC PATIENTS?
Objective: The objective of this study is to evaluate the antihypertensive drug usage and dosage differences between geriatric and non-geriatric diabetics with reference to the duration of hypertension and creatinine cle...