Phytochemical Screening, HPTLC and GCMS Profile of Acacia catechu (L.f) Willd Hydroethanolic Leaf Extract
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 1
Abstract
The plant Acacia catechu (L.f) Willd hydroethanolic leaf extract was subjected to qualitative and quantitative phytochemical screening, HPTLC and GCMS analysis. The phytochemical analysis revealed the presence of carbohydrates, proteins, flavonoids, total phenolics, glycosides, total free amino acids, tannins, alkaloids and thiols. The secondary metabolites like flavonoids, tannins and total phenolics were quantified as 82μg/ml, 67.5μg/ml and 42.5μg/ml. The HPTLC analysis of the leaf extract revealed the presence of flavonoids, tannins, phenolics and alkaloids. GCMS chromatogram showed sixty seven peaks which indicated the presence of thirteen phytochemical constituents. The major constituents were Furo[2,3-d] Pyrimidine-4,6 [5H,7H]-dion (10.20%), 2-Methyl-1,2,3,4-tetrahydro-beta-carboline(16.26%), Pthalic acid, butyl 2- pentyl ester (4.35%), Butylphosphonic acid and di(4-methoxy benzene) (14.82%). These results indicated that the Acacia catechu (L.f) Willd contain various bioactive components with wide range of medicinal properties, justifying the use of this plant to treat various diseases.
Authors and Affiliations
B. Ramesh, V. Jayabharathi
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