Phytochemical Screening, Total Phenolic and Antioxidant Activity of Crude and Fractionated Extracts of Cynomorium coccineum Growing in Saudi Arabia
Journal Title: European Journal of Medicinal Plants - Year 2016, Vol 11, Issue 4
Abstract
Aim: This study was carried out to determine the Total phenolic and antioxidant activity of crude and fractionated extracts of Cynomorium coccineum growing wild in Saudi Arabia. Methodology: Phytochemical analysis of the different extracts obtained from C. coccineum was carried out by Siddiqui et al procedure and the Total phenolic content of the extracts were assessed by the Folin-Ciocalteau’s method while the antioxidant activities were determined by two different assays, namely scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) cation radicals methods. Results: Phytochemical screening of C. coccineum revealed the presence of alkaloids, anthraquinones, glycosides, flavonoids, saponins, tannins and terpenoids in all different extracts. In the present study, the butanol extract exhibited the highest total phenolic content (201.36±7.44 mg of GAEs/g of extract) and showed the strongest antioxidant activity in both, the DPPH radicals and ABTS methods. The antioxidant activity of the butanol extract for DPPH scavenging assay was 95.20%±3.48% (IC50 0.0056 mg/mL) while for ABTS radical scavenging assay was 99.07±0.37 (IC50 0.006 mg/mL) at concentration 0.4 mg/mL. Conclusion: The high phenolic content in the polar extract accounts for the high antioxidant potential in comparison to non-polar extract. The results of this study substantiates the role of these plant as natural sources of antioxidants.
Authors and Affiliations
Jehan Al-humaidi
Keywords
Related Articles
- EP ID EP349633
- DOI 10.9734/EJMP/2016/18820
- Views 97
- Downloads 0
A Preliminary Ethno-medical Survey of Plants Used in the Traditional Management of Cancer and Related Diseases amongst Tarok People of Plateau State, North-Central Nigeria
Aim of the Study: To document medicinal plant used in the treatment of cancer and related diseases amongst Tarok people of Plateau State, North-Central Nigeria. Study Design: Ethno medical survey and medicinal plant docu...
In vitro Anthelmintic Activity of Bidens pilosa Linn. (Asteraceae) Leaf Extracts against Haemonchus contortus Eggs and Larvae
Haemonchus contortus is a major health and welfare problem for small ruminants, responsible for economic losses through reduced productivity and increased mortality. The in vitro efficacy of Bidens pilosa was determined...
Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Tectona grandis Leaves, Using Experimental Design
Aims: This study aims to apply central composite design to optimize ultrasound-assisted extraction conditions in order to maximize simultaneously total polyphenols and antioxidant activity from aqueous leaves extract of...
Phytochemical Screening of Isolated Compounds from Nymphaea nouchali Burm.f. Flowers
Objective: To learn the phytochemical screening of various compounds from different extracts of Nymphaea nouchali Burm.f flowers. Materials and Methods: Nymphaea nouchali flowers were extracted with different solvents (n...
Phytochemical Screening, Antifungal and Antibacterial Effect of Zanthoxylum zanthoxyloides and Zanthoxylum macrophylum Used in Traditional Medicine in Yamboro (Central African Republic)
The knowledge of traditional medicine has always guided the search for new cures. Zanthoxylum zanthoxyloides and Zanthoxylum macrophylum, (Rutaceae) are used as medicinal plants in Central African Republic for bacteria a...